ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The kinetic study of extracellular nuclease of the strainSerratia marcescens is reported. Mathematical description of a substrate inhibition observed in the DNA decomposition ofSerratia nuclease is given. By computer process, the following parameters of the kinetic model have been identified: Ks, Km and Vmax values characteristic of the noninhibited decomposition process and rate constants k1, k−1 and k2 of the elementary steps of the enzyme reaction. The effect of the temperature and pH on the reaction rate and specific rate constants has been studied. A new subtype of substrate inhibition is suggested.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00930876
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