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  • 1975-1979  (3)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Plasmids in Pseudomonas (1976)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 10 (1976), S. 7-30 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.10.120176.000255
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal mobilization from a recA mutant of Pseudomonas putida (1979)
    Springer
    Molecular genetics and genomics 176 (1979), S. 151-154 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A methyl methane sulfonate sensitive mutant of P. putida strain PpG1 is also extremely sensitive to UV-rays, compared to parent wild type cells. This mutant behaves typically as recombination less (recA) mutants of Escherichia coli and Pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. Sex factor plasmids such as K-XYL or TOL can mobilize chromosomal genes equally well both from recA + and recA801 donor cells, suggesting that host recombination functions are not necessary for mobilization of chromosomal genes by such plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334307
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Mapping of Pseudomonas putida chromosomal genes with a recombinant sex-factor plasmid (1977)
    Springer
    Molecular genetics and genomics 157 (1977), S. 231-237 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transfer of the non-conjugative xylene-degradative plasmid, XYL, mediated by the sex factor plasmid K, from Pseudomonas Pxy to P. putida strain PpGl results in the formation of XYL+ K+ PpGl exconjugants. Isolation of plasmid DNA from exconjugants, transductants and transformants and their electron microscopic characterization suggest that they occur predominantly as plasmid co-integrates with a varying number of copies of XYL on K. Free forms of XYL and K also occur at low frequency. Growth of cells harboring such recombinant XYL-K plasmids with xylene (or intermediates such as meta-toluate) ensures the retention of K. A membrane filter mating technique has been developed, using PpGl cells harboring XYL-K plasmids as donors and K- auxotrophs as recipients. The time of entry of a number of individual chromosomal markers has been precisely determined, and a tentative map of the P. putida chromosome has been constructed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268659
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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