Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1975-1979  (4)
  • 1
    Electronic Resource
    Electronic Resource
    UPTAKE OF GLUCOSE ANALOGUES BY RAT BRAIN CORTEX SLICES: A KINETIC ANALYSIS BASED UPON A MODEL (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 27 (1976), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— In slice preparations the exchange of dissolved substances between cells and incubation medium is delayed by diffusion through the extracellular space. The delay may seriously interfere with the study of membrane transport in terms of unidirectional fluxes across the cell membranes. A three-compartment serial model has been developed to describe exchange between slice and incubation medium. By aid of this model it is shown that the diffusion delay prevents determination of unidirectional fluxes for the two non-metabolizable glucose analogues 3-O-methylglucose and α-methyl-glucosidc. The membrane transport of the slowly transported α-methylglucoside can however be examined by aid of the model whereas the transport of 3-O-methylglucose is so rapid that it can not be examined with respect to Vmax Km and Kr. An attempt to determine these parameters will result in falsely large values which reflect extracellular diffusion and not membrane transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1976.tb12254.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    UPTAKE OF GLUCOSE ANALOGUES BY RAT BRAIN CORTEX SLICES: Na+-INDEPENDENT MEMBRANE TRANSPORT (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 27 (1976), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— The glucose analogues 3-O-methyl-D-glucose and α-methyl-D-glucoside were not metabolized in brain tissue.The uptake of these two sugars into the intracellular compartment of brain cortex slices was investigated using media with normal and low Na+ concentration (replacement of all NaCl with choline Cl). The cellular transport was not Na+-dependent. The transport mechanism clearly distinguished between the two sugars in both normal and low Na+ media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1976.tb12255.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    UPTAKE OF GLUCOSE ANALOGUES BY RAT BRAIN CORTEX SLICES: MEMBRANE TRANSPORT VERSUS METABOLISM OF 2-DEOXY-d-GLUCOSE (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 29 (1977), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract— —The uptake of the glucose analogue 2-deoxy-d-glucose by rat brain cortex slices was studied in order to compare the rate of membrane transport with the rate of phosphorylation in the concentration range 5–12 mM-glucose plus 0.5–15 mM-2-deoxy-glucose. The comparison was carried out by fitting a model of the brain slice to uptake data and by determination of 2-deoxy-glucose and 2-deoxy-glucose-6-phosphate by ion exchange chromatography.The rate of membrane transport exceeded the rate of phosphorylation by at least one order of magnitude. The membrane transport was so rapid that the extracellular diffusion became rate limiting for the uptake. The membrane transport could therefore only be determined as a minimum value and it was not possible to determine unidirectional flux across the cell membranes (initial rate). Accordingly, characterization of the membrane tranport with respect to maximal transport rate and affinity was not possible. The phosphorylation reaction, however, was so slow that it was accessible for exact determination and only the phosphorylation reaction was responsible for the fact that the cellular uptake of 2-deoxy-glucose was of the Michaelis-Menten type, thus emphasizing the importance of dissociation between membrane transport and metabolism when transport is studied of a substance which can undergo metabolism.The data indicate that glucose transport across glial and neuronal membranes is not rate limiting for glucose metabolism of brain tissue in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1977.tb09610.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Uptake of inulin by cells in rat brain cortex (1977)
    Springer
    Experimental brain research 28 (1977), S. 37-50 
    Details
    ISSN: 1432-1106
    Keywords: Brain cortex slices ; Membrane transport of macromolecules ; Inulin space
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Light and electron microscope autoradiography indicated that 3H-labelled inulin was taken up by neurons and glia cells of rat brain cortex in vitro. The mechanism, by which inulin passed the cell membranes, was studied by comparing the transport of inulin (molecular weight 5000) with the transport of dextran (molecular weight 75000). The half-time for the cellular in- and efflux for the two molecules was the same although their diffusion coefficients differed by a factor of 4–5. The transport mechanism was therefore interpreted as bulk transport, and vesicular transport is suggested. Efflux of inulin from brain cortex exposed to inulin in vivo indicated that cellular uptake of inulin also occured in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237084
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...