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Total protein release from barley aleurone layers as a bioassay for gibberellins (1978)

Mapelli, S. ; Ranieri, A. M.

Springer

Planta 142 (1978), S. 37-40

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ISSN: 1432-2048

Keywords: Aleurone ; Gibberellin ; Hordeum ; Protein release

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The effect of gibberellic acid on the secretion of proteins from barley (Hordeum vulgare L.) aleurone layers has been investigated for its suitability as a gibberellin bioassay. Concentrations from 10−4 μg/ml to 100 μg/ml of GA3 resulted in the release of proportionally increasing amounts of total protein. The release of proteins is not affected by indoleacetic acid and kinetin. This method has been applied and compared with the α-amylase assay for the estimation of gibberellin in extracts of tomato fruits and maize seedlings.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00385117

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