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  • 1975-1979  (2)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12 (1978)
    Springer
    Molecular genetics and genomics 164 (1978), S. 1-8 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A temperature-sensitive mutation in the dnaJ gene of Escherichia coli K12 is described which affects replication of the bacterial DNA. The gene is located adjacent to the dnaK gene described previously (Saito and Uchida, 1977). The physical and functional organization of the dnaJ-dnaK region was studied in detail by analyzing the heteroduplexes and functions of various deletion mutants of λdnaJdnaK, a transducing phage carrying both of the dna genes. The sizes of dnaJ and dnaK cistrons were estimated to be at most 1.2±0.5 and 2.1±0.4 kilobases, respectively. In vivo expression of the dnaJ function by various deletion phages indicated that the dnaK and dnaJ cistrons were transcribed from a promoter located at the head of the dnaK cistron, dnaJ being downstream to dnaK. Presence of a weak promoter which reads only the dnaJ cistron was also suggested. A simple method for isolating independent deletion mutants of phage λ was described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267592
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A transducing λ phage carrying grpE, a bacterial gene necessary for λ DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19) (1978)
    Springer
    Molecular genetics and genomics 165 (1978), S. 247-256 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A grpE mutation of Escherichia coli K12, which blocks DNA replication of the phage λ (Saito and Uchida, 1977), was mapped at 56 min on the standard genetic map. A transducing λ phage, λgrpE22, carrying the wild type allele of the grpE gene was constructed in vitro. Structures of λgrpE22 and its viable deletion derivatives were determined by electron microscopic analyses of appropriate heteroduplexes. Proteins coded by the bacterial DNA incorporated into the transducing phages were detected by two-dimensional gel electrophoresis. The results showed that the product of the grpE gene is a weakly acidic protein of molecular weight 24,000. Structural genes for two ribosomal proteins, rplS (L19) and rpsP (S16) were also shown to be carried by λgrpE22.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332523
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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