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  • 1970-1974  (5)
  • 1910-1914
  • Biochemistry and Biotechnology  (4)
  • Light and electron microscopy  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructure de l'œuf de Triatoma infestans Klug (1973)
    Springer
    Cell & tissue research 138 (1973), S. 113-132 
    Details
    ISSN: 1432-0878
    Keywords: Eggs ; Insects (Triatoma infestans) ; Embryogenesis ; Envelopes, Permeability ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The thick rigid chorion of the egg of Triatoma secreted by the follicle cells shows two porous layers: an aerial layer in the exochorion, an alveolar one in the endochorion. The anterior part of the eggshell is closed up by an operculum which is heaved up by the hatching larva. The operculum has no alveolar layer. The air enters through the numerous holes of the shell surface into the aerial layer and through the micropyles into the alveolar layer. The egg has no respiratory plastron. The follicle cells produce also a vitelline envelope whose structure shows a rapid condensation at fertilization time. During its development the embryo secretes two layers: serosal and embryonic cuticle. At high humidities, at low temperatures the egg is able to increase its weight during the early stages of embryogenesis, and this increase stops when the serosal cuticle is secreted. In a dry atmosphere the egg loses water but can develop if the temperature is higher than 20°C. The little permeability of the egg is related to the structure of its envelopes. The chorion and the vitelline envelope prevent the water from getting out of the egg. The serosal cuticle seems to be opposed to the penetration of the water into the egg. The role of the embryonic cuticle is probably limited in the transit of water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307082
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dialysis fermentation. III. Anomalous inhibition of threonine biosynthesis in an auxotrophic mutant of Escherichia coliArticle No. 4979, Michigan Agricultural Experiment Station. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 603-613 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dialysis was attempted as a means to alleviate the product-activated controls presumed to limit the formation of threonine by an auxotrophic mutant of Escherichia coli strain W. The occurrence of inhibition rather than enhancement of yields by dialysis was traced to the fact that threonine actually was not inhibiting its own synthesis. Instead, α,ε-diaminopimelic acid became depleted, but the imbalance could not be corrected by exogenous replacement.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260120409
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dialysis fermentation. I. Enhanced production of salicylic acid from naphthalene Pseudomonas fluorescensArticle No. 4977, Michigan Agricultural Experiment Station. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 577-589 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The conversion of naphthalene to salicylic acid by Pseudomonas fluorescens was studied as a model for dialysis fermentation. In a demonstration experiment, the continual removal of the product by dialysis and by intermittent replenishment of the dialysate reservoir caused cyclical changes in the concentrations of viable cells and product. The cumulative total amount of both cell mass and salicylate, however, continued to increase steadily until the experiment was terminated after 15 days. At this time the rate of product formation was highest and still increasing, although less than 10% of the cells were viable. The terminal amount of salicylate was about 20-fold greater than the maximum reached in the control fermentation, and was calculated to be 2.6-fold more productive even if the control were optimally recycled. Methods were projected to achieve still further improvements.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260120407
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The relationship of substrate, growth rate, and maintenance coefficient to single cell protein production (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 117-127 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Various organic compounds were assessed as potential substrates for single cell protein production. Substrate evaluation was based on the costs associated with the substrate, oxygen, and heat yield coefficients: Ysub, Yo, and Ykcal, respectively. Yo, and Ykcal, were calculated from experimental values of Ysub, and from the elemental composition of bacterial cells. The dependence of the yield coefficients on the specific growth rate (μ) and maintenance coefficient (m) also was assessed. The analysis disclosed that m caused two- to threefold variations in the yield coefficients as μ was increased from 10% to 100% of μmax. The effect of different m values at constant specific growth rates also was determined. The value of m had a significant effect on the yield coefficients even at high specific growth rates. Assignment of cost factors to the yield coefficients provided an estimation of the impact m and μ on biomass production costs.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150109
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Dialysis fermentation. II. Kinetic analysis of salicylic acid production via cell growth and maintenanceArticle No 4978, Michigan Agricultural Experiment Station. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 591-601 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A kinetic analysis was made of the relationship between salicylate production from naphthalene and growth of Pseudomonas fluorescens in semicontinuous dialysis culture. The specific rates both of product formation and growth initially were increased by the diffusional withdrawal of salicylate, but subsequently were reduced to low levels despite continued salicylate removal. Productivity and growth were correlated by the Luedeking-Piret equation in an initial nondialysis period and in the early stages of dialysis fermentation, when specific growth rates exceeded. 005 hr-1. Below this level of growth at later stages of dialysis fermentation, the specific production rate was correlated only with total cell mass by a proportionality constant of .035 hr-1, which was attributed to maintenance metabolism. Maintenance accounted for about 84% of the total salicylate produced, while growth-associated metabolism accounted for the remainder.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260120408
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    Paper (German National Licenses)
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