Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1970-1974  (3)
  • Analytical Chemistry and Spectroscopy  (2)
  • Chromogranin  (1)
  • Chromogranin A
  • 1
    Electronic Resource
    Electronic Resource
    Bovine adrenal medulla: Synthesis and secretion of radioactively labelled catecholamines and chromogranins (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 271 (1971), S. 193-203 
    Details
    ISSN: 1432-1912
    Keywords: Adrenal Medulla ; Chromogranin ; Catecholamines ; Synthesis ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. 3H-leucine and3H-tyrosine were added to the perfusion medium of isolated bovine adrenal glands in order to study the synthesis and secretion of radioactively labelled catecholamines and proteins. 2. Various times after the injection of the labelled amino acids the soluble proteins of the microsomal fraction were isolated and subjected to polyacrylamide gel electrophoresis in three different buffer systems. The distribution of protein-bound radioactivity within the gels was determined. Most of the label was found to be confined to proteins which behaved like the specific soluble proteins of chromaffin granules, i.e. the chromogranins. 3. Stimulation of the adrenal gland with carbachol induced a release of catechol-amines, protein, and protein-bound radioactivity. In the absence of Ca2+ the secretion of all these components was abolished. The highest specific radioactivity of the proteins secreted upon stimulation was reached 4 h after the injection of the labelled precursors. The labelled proteins, secreted upon stimulation, could be identified as chromogranins. 4. Carbachol induced the release of highly labelled catecholamines after the injection of3H-tyrosine. The highest specific radioactivity of these catecholamines was already observed at the first stimulation with carbachol i.e. 30 min after3H-tyrosine. 5. These results demonstrate that in isolated bovine adrenal glands radioactively labelled chromogranins and catecholamines can be synthesised and can be secreted upon stimulation with carbachol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00998580
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Field desorption mass spectrometry of amino acids (1972)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 6 (1972), S. 655-660 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectra of some amino acids have been studied using the field desorption method. All amino acids yield molecular or quasi-molecular ions, even in the case of arginine and cystine, where these ions cannot be detected with the electron-impact (EI) or chemical ionization (CI) methods. The fragmentation is reduced as compared to EI, CI and FI.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210060605
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Field desorption mass spectrometry with indirect heating of the emitter (1973)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1973), S. 1007-1009 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In field desorption mass spectrometry it is in many cases more expedient to heat the field anode indirectly by i.r.-radiation instead of the usual method of electric heating to achieve higher relative molecular ion intensities.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210070812
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...