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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 22 (1972), S. 42-57 
    ISSN: 1432-0533
    Keywords: Vincristine ; Brain ; Spinal Ganglia ; Mouse Fetus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Brain and spinal ganglia of mouse fetuses were examined under the light- and electon microscope 4 to 24 h after a 5 mg/kg dosage of vincristine. The following changes were observed: 1. In many proliferating cells, an inhibition of mitosis during metaphase could already be found after 4 h. 2. Numerous interphase cells develop damaged nuclear structures and organelles at the end of the 24 h period. On the one hand mitotic inhition can be demonstrated only in the proliferating paraventricular zones of the brain and also thinly scattered in the spinal ganglia. On the other hand cells demonstrating the typical pattern of damage to nucleus and organelles can be found diffusely spread through the whole cortex and spinal ganglia. The chromatin is clumped together in the nuclei of these cells, it is also extremely electron dense and arranged in wide threads. The perinuclear cistern is very widened and has often disintegrated into vesicles. Hereby wide connections between caryo- and cytoplasma develop. The number and size of the rough ER is decreased, often puffed up and vesicularly disintegrated. The quantity of free ribosomes has also decreased and polysomes are scarcely seen. The other cell organelles are often swollen. The connection between vincristine and the ultrastructural changes can be partly understood on the basis of the biochemical findings.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 111 (1970), S. 572-585 
    ISSN: 1432-0878
    Keywords: Brain ; Intraventricular cellular processes ; Ependyma ; Lateral ventricle ; Synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ependym und subependymale Strukturen der Seitenventrikel von Katzen aus dem Bereich des Nucleus caudatus und des Corpus callosum wurden nach Perfusionsfixierung elektronenmikroskopisch untersucht. Dort findet sich eine Reihe von Neuritenanschnitten, deren kolbenförmige Auftreibungen leere Vesikel, “dense core granula” und Mitochondrien enthalten. Neben diesen neuronalen Elementen werden auch Zellfortsätze nichtneuronaler Natur im Ventrikellumen beobachtet, die Ependym- und intraventrikulären Zellen entstammen. An umschriebenen Stellen nehmen diese kolbig aufgetriebenen Fortsätze desmosomenartige Kontakte mit dem Ependym auf. An diesen Orten umgeben Mikrovilli die Fortsätze korbartig. Ob es sich bei diesen Kontakten um synapsenartige Strukturen handelt, oder um „Rezeptoren“ für unbekannte chemische Stoffe, wird diskutiert. Die Kontakte sind unabhängig von der Form der Ependymzellen und davon, ob diesen graue oder weiße Substanz unterliegt. Zwischen und in den Ependymzellen finden sich — bedingt durch den unterschiedlichen Gehalt an Zellorganellen — drei verschiedene Arten von Fortsätzen. Die Zahl der neuronalen Fortsätze in diesem Bereich ist sehr viel kleiner als im Ventrikellumen.
    Notes: Summary Ependyma and subependymal regions of the lateral ventricles of cats (area of Nucleus caudatus and Corpus callosum) were fixed by perfusion and investigated electronmicroscopically. Intraventricular axons showing a beaded shape with smaller and thicker parts were found. The varicosities contain empty vesicles, dense core granules and mitochondria. Beside those certainly neuronal elements there exist intraventricular cell processes originating from ependymal cells and free cells. The neuronal processes make desmosome-like contacts with the ependyma. These regions of contact are surrounded by basket-like arranged microvilli. It is discussed, whether the contacts function like synapses or as receptors for a substance, which is still unknown. Contacts do not depend on the shape of ependymal cells. They exist with the ependyma of Nucleus caudatus (grey substance) as well as with the ependyma of Corpus callosum (white substance). Because of the different contents of cell-organelles one can differentiate three different sorts of processes situated inter- or intracellularly in the ependyma. Neuronal processes within the lateral ventricles are more numerous than those found within the ependymal layer.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 156 (1974), S. 89-102 
    ISSN: 1432-0878
    Keywords: CNS tissue culture ; Blood vessels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Explants from spinal cord, brain stem, cerebellum and cerebral cortex of 5–18 weeks old human embryos and newborn rats were cultivated for 2–30 days. Most of the blood vessel fragments in the explant showed a rather normal structure of their wall even after 30 days in vitro. However, the number of small vessels was reduced. The lumen of most vessels was not collapsed and in some cases filled with cell debris. The endothelial cells remained connected by terminal bars, contained many vesicles and vacuoles of varying size and osmiophilic inclusions of cell debris. While in large vessels the endothelium was always present, in some small vessels near the edge of the explant it was absent. Here, glial endfeet made contact with a basal lamina, which enclosed varying amounts of cell debris. Pericytes were rarely observed after more than 10 days in vitro. Although the perivascular astroglial sheath disintegrated during the first few days in vitro, it was reconstructed and, after about 10 days in vitro, became more complete and differentiated than it was at the time of explantation. Many glial endfeet contained aggregated osmiophilic inclusions. At these sites the perivascular glial sheath and the adhering basal lamina often were discontinuous. The basal laminae were well developed, but disintegrated where the glial sheath was discontinuous. It is concluded that in vitro vessel fragments may be preserved without normal function and basal laminae survive without endothelium.
    Type of Medium: Electronic Resource
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