ISSN:
1432-1912
Keywords:
Botulinum A
;
Tetanus
;
Neurotoxin
;
Hemagglutinin
;
Iodine Labeling
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. Labeling of crystalline botulinum A toxin has been done with 125I by aid of the chloramine T method. The neurotoxic component is well preserved, whereas the hemagglutinin undergoes physicochemical alterations. Neither with labeled nor with unlabeled toxin, hemagglutinating power parallels the main protein peak. 2. Neurotoxin, homogeneous in gel filtration, is bound to synaptosomes from rat brain. Cold toxin competes with labeled toxin, and antitoxin or neuraminidase partially remove the bound neurotoxin. 3. Upon intramuscular injection, some radioactivity is recovered in the respective parts of the spinal cord. Antitoxin prevents the ascent. The similarities between tetanus and botulinum A neurotoxins are stressed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00500611
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