ISSN:
1432-0827
Keywords:
Human
;
Dentine
;
Glycosaminoglycan
;
Chondroitin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Abstract The glycosaminoglycans of root dentine from permanent human teeth were studied. The tissue was digested with papain and the glycosaminoglycans were precipitated with cetylpyridinium chloride and recovered by centrifugation. A small quantity of keratan sulphate was recovered from the supernatant after fractionation on cetylpyridinium chloride and Ecteola-cellulose. The cetylpyridinium chloride-precipitated glycosaminoglycans were studied by infra-red spectrophotometry, cetylpyridinium chloride cellulose chromatography and electrophoresis on cellulose acetate. Individual hexosamines were determined on an autoanalyzer. The results indicate that chondroitin-4-sulphate is the major glycosaminoglycan and that chondroitin-6-sulphate, hyaluronic acid, dermatan sulphate and a nonsulphated galactosaminoglycan are also present in minor quantities.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02008211
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