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  • 1
    Electronic Resource
    Electronic Resource
    Cytology, cytochemistry and ultrastructure of glucagon-secreting cells (1970)
    Springer
    Acta diabetologica 7 (1970), S. 542-556 
    Details
    ISSN: 1432-5233
    Keywords: A-cell ; α-granules ; Cytochemistry ; Cytology ; Enteroglucagon ; Glucagon ; Glucagonsecreting cells ; Pancreatic islets ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les principales méthodes morphologiques pour la détection des cellules A ont été examinées et leur sélectivité a été amplement discutée. Une attention particulière a été dédiée aux méthodes pour les indoles et aux observations immunoistochimiques et ultrastructurelles. Les Auteurs rapportent les données immunoistochimiques concernant les cellules A telles que cellules dotées d'activité glucagono-sécrétive, ainsi que les observations ultrastructurelles qui font penser à une éterogénéité des granules α. Actuellement il n'existe pas des preuves morphologiques définitives de l'existence de cellules A gastro-intestinales.
    Abstract: Resumen Se examinan las metódicas morfológicas para la evidenciación de las células A y se discute ampliamente la selectividad. Se da particular importancia a los métodos al indol y a las observaciones inmunohistoquímicas y de microscopia electrónica. Se refieren los datos inmunohistoquímicos relativos a las células A tales como células secretoras de glucagon y las observaciones electrónmicroscopicas que hacen pensar en una heterogeneidad de los gránulos α. Actualmente no tenemos pruebas morfológicas sobre la existencia de células A gastrointestinales.
    Notes: Riassunto Vengono prese in esame le principali metodiche morfologiche per la evidenziazione delle cellule A e ne viene ampiamente discussa la selettività. Particolare rilievo viene dato ai metodi per gli indoli e alle osservazioni immunoistochimiche e di microscopia elettronica. Vengono riferiti i dati immunoistochimici relativi alle cellule A quali cellule secernenti il glucagone, nonché le osservazioni ultrastrutturali che fanno pensare ad una eterogeneità dei granuli α. Attualmente non possediamo prove morfologiche definitive dell'esistenza di cellule A gastro-intestinali.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01556805
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Light and electron microscopic identification of the histamine-storing argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals (1971)
    Springer
    Cell & tissue research 118 (1971), S. 68-84 
    Details
    ISSN: 1432-0878
    Keywords: Argyrophil cells ; Gastric hormones ; Carcinoids ; Ultrastructure, cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A peculiar type of cell, the ECL cell, accounts for a large portion of nonenterochromaffin endocrine cells in the gastric oxyntic glands of several mammals, including rat, mouse, guinea pig, rabbit, cat, dog, pig, and man. The ECL cell is characterized mainly by its secretory granules with irregular cores heavily reacting to Grimelius' silver, Sevier-Munger silver and phosphotungstic acid, while failing to react, in all species but the cat and rabbit, to Masson's argentaffin method. In the rat and mouse, the ECL cell seems to correspond exactly to the argyrophil non-argentaffin histamine-storing “enterochromaffin-like” cell of Håkanson and Owman (1967); in the remaining species, ECL cells seem to account for only a part of the gastric argyrophil “enterochromaffin-like” cells described by Håkanson and coworkers. Besides sporadic amines, ECL cell granules store non-amine components, whose possible nature is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331767
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Endocrine cells of the human gastric mucosa (1971)
    Springer
    Cell & tissue research 118 (1971), S. 49-67 
    Details
    ISSN: 1432-0878
    Keywords: Enterochromaffin cells ; Argyrophil cells ; Gastrointestinal hormones ; Ultrastructure ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By light and electron microscopy investigation of the human gastric mucosa five types of ultrastructurally different endocrine cells have been detected: 5-hydroxytryptamine storing enterochromaffin (EC) cells, gastrin storing G cells, and functionally undefined ECL, D and D1 cells. By direct application of Masson's argentaffin reaction as well as of Sevier-Munger's and Grimelius' argyrophil method to electron microscopy specimens, selective deposition of silver grains upon the endocrine granules of such cells was obtained. In particular, only EC cell granules reacted to the argentaffin method, granules of both EC and ECL cells heavily reacted to Sevier-Munger's technique, granules of EC, ECL, G and D1 cells reacted to Grimelius' technique, while D cell granules failed to react either to argentaffin or argyrophil methods. By the application of the same silver methods to paraffin sections as well as by other selective staining methods for endocrine granules (5-hydroxytryptamine techniques, lead-haematoxylin, HCl-basic dye method), at least four of the above cell types were also identified under light microscope. This opens the way for extensive studies of such cells in conventional histologie specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331766
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    Paper (German National Licenses)
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