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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 28 (1972), S. 1087-1089 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Erstmals werden mit Hilfe der Gefrierätzung die Synapsen im Ductus deferens dargestellt, und es kamen dabei partikuläre Auflagerungen auf das Axolemm erstmals zur Darstellung.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 59 (1972), S. 125-126 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 60 (1973), S. 208-208 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 79 (1973), S. 148-156 
    ISSN: 1432-1335
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Calcium-phosphate-phospholipid complexes have been isolated from experimental tumors. The Chromatographie analysis indicates that most of the phosphatidylserine and a part of the phosphatidylethanolamine from the phospholipid of the tumor are bound to calcium in a complex. The proportions of calcium and phosphorus in the complexes show variations related to the type of tumor and to its age.
    Notes: Zusammenfassung Aus künstlich erzeugten Tumoren wurden Calcium-Phosphat-Phospholipid-Komplexe isoliert. Die chromatographische Analyse zeigt, daß der größte Teil des Phosphatidylserins und ein Teil des Phosphatidyläthanolamins der im Tumor enthaltenen Phospholipide sich mit Calcium verbinden. Das Verhältnis von Calcium und Phosphor in diesen Komplexen schwankt in Abhängigkeit von Typ und Alter des Tumors.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 577-586 
    ISSN: 1432-0878
    Keywords: Node of Ranvier ; Axonal-glial junctions ; Cat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The paranodal zone of myelinated nerve fibers from the CNS of cats has been studied by means of the lanthanum technique and after freeze-etching. Special interest was given to the axonal-glial zones of contact in this region. Examination of the membranes of the paranodal pockets and the axolemma revealed that the membrane thickenings which occur between the adjacent membranes actually consist of bands. The bands show a specific pattern of diagonal running subunits 10 nm apart. These substructures of the axonal side of the membrane contacts protrude outwards, i.e. towards the glial membrane, where they fit into depressions on the glial membrane surface. This arrangement in form of a “zip-fastener” makes the whole structure very flexible. The subdivision of the bands was identified with both techniques. The different membrane aspects obtained by the freeze-etching technique are discussed in respect to the splitting theory, and a hypothetical model of this membrane specialization is presented.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 149 (1974), S. 121-135 
    ISSN: 1432-0878
    Keywords: Astrocytic membranes ; Cat ; Orthogonal particle-complexes ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fixed and unfixed astrocytic membranes from the CNS of the cat were studied by means of the freeze-etching technique. A variable number of gap junctions was detected in astrocytic membranes. They are characterized by the well known hexagonal composition of their subunits. Besides this type of highly ordered membrane-bound particles, a second one was found. It is composed of four single particles (diameter 5 nm) which form an orthogonal subunit with a side length of about 10 nm. These membrane-associated orthogonal particle complexes (MOPC) could be observed in different stages of aggregation and expansion. They reveal an accumulation in membranes of the marginal glia layers and in the perivascular astrocytic end-feet. Unfixed, glycerol treated membranes, however, do not show these structures. After glycerol treatment of the unfixed membranes by immersion, the MOPC disintegrate to single particles which form clusters of various extension. The clustering phenomenon is dependent on the length of the time of exposure to glycerol. Shortening of the glycerol treatment by intravasal perfusion of the cryoprotectant agent causes an decrease of the clusters. Fragments and transient forms of the MOPC become visible. By variation of different physico-chemical parameters of the washing solution a similar effect on the MOPC was not achieved. The discussion deals with probable functional aspects of the MOPC. They are considered to act as membrane-bound functional multienzyme complexes which a) might play a role in mediating transmembrane passage of metabolites, or b) are essential for CSF control mechanisms, or c) have a functional relation to the nexus.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 565-576 
    ISSN: 1432-0878
    Keywords: Central myelin ; Junctions ; Cat ; Freeze-etching ; Lanthanum impregnation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In male and female cats central myelin was studied using the freeze-etching technique, and after impregnation with lanthanum as a tracer. The freeze-fractured and etched faces of the myelin reveal membrane formations which in many respects resemble tight junctions. The formations consist of parallel rows of particles on one half of the membranes matched by corresponding furrows on the other membrane half. These structures occur across the width of the myelin sheath and are most common in that part of the myelin underlying the external cytoplasmic loop. They are interpreted as correlates of the radial component of the i-line which occurs in cross-sections of central myelin after potassium permanganate staining. In the paranodal zone the lanthanum-impregnated myelin displays punctate fusions between the outer leaflets of the oligodendrocytic membranes. These findings strongly suggest that tight junctions are a common feature in the central myelin. The arrangement of the membrane fusion in relation to the elements of the lamellar complex and their functional significance is discussed.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 137 (1973), S. 111-124 
    ISSN: 1432-0878
    Keywords: Ductus deferens ; Rats and mice ; Vegetative nerve fibres ; Membrane structure ; Freezeetching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Präterminale und terminale vegetative Nervenfasern aus dem Ductus deferens von Ratten und Mäusen wurden mit Hilfe der Gefrierätztechnik untersucht. Die Ultrastruktur der Axone in den Abdrücken stimmt mit dem Bild aus konventionell gewonnenen Präparationen im wesentlichen überein. Die Untersuchung ergab eine Zunahme membrangebundener Partikel in terminalen Abschnitten an der (+)-Seite des Axolemms. An freien Axolemmbereichen sowie an myoneuralen Kontakten treten diese Partikel, die einen Durchmesser zwischen 6–10 nm besitzen, in Form kleinflächiger Ansammlungen oder in größeren, plaqueförmigen Aggregationen auf. Membranfusionen von synaptischen Vesikeln mit dem terminalen Axolemm im Sinne eines herkömmlichen Exozytosevorganges konnten im untersuchten Material nicht nachgewiesen werden.
    Notes: Summary Preterminal and terminal vegetative nerve fibres of the ductus deferens of rats and mice were studied with the freeze-etching technique. The ultrastructure of the axons in freeze-fractured and etched replicas is in agreement with that obtained by conventional electron microscopical techniques. An increase in the number of membrane-bound particles was observed in the terminal region of the (+)-side of the axolemma. These particles, wich have a diameter of 6–10 nm, tend to aggregate in either small or large, plaque-like accumulations at parts of the axolemmata, which are free from Schwann cells and at myoneural junctions. The possible function of these particle-aggregations is discussed. Fusions of synaptic vesicles with the terminal axolemma in the form of a conventional exocytotic process could not be detected in this material.
    Type of Medium: Electronic Resource
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