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  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 36 (1973), S. 115-121 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiographic experiments are described in which low and high molecular radioactive compounds with different types of radiation are used. The effect of the following procedures on the spread of grains were studied: 1) cutting-direction, 2) diffusion of molecules at cryostate temperatures, 3) the type of radiation, 4) time lapse between dissection and freezing of the tissue. It is suggested that the resolution with 125Iodine-labeled compounds is worse than with tritium-labeled compounds, owing to different radiation.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 39 (1974), S. 345-349 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In previous autoradiographic model experiments with diffusible substances the resolution of 125iodide appeared to be worse than that of tritiated compounds. This could be attributed to a difference in type of radiation (resulting in overradiated autoradiographs made with 125iodide) or to a difference in diffusion. In our present experiments autoradiographs of trachea segments filled with high and low quantities of free 125iodide and albuminbound 125Iodine were compared and the resolutions estimated. Overradiation (high quantities of the radioactive substances) did not influence the resolution, but enlarged the distance from the source to background level. Irrespective of the quanatity of radioactivity, a significant difference in resolution was found between 125iodide and iodinated (125I) albumin. Moreover, the resolution of iodinated (125I) albumin was the same as that of tritiated compounds. The conclusion was made that the difference in resolution between 125iodide and tritiated compounds was due to a greater diffusion of iodide ions during the experimental procedure.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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