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Isolation of nuclei from rice tissue grown in suspension culture (1974)

App, A. ; Granados, R. R.

Springer

Planta 120 (1974), S. 201-205

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ISSN: 1432-2048

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00384931

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