ISSN:
1573-4927
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract RNA from 6-day preimplantation rabbit blastocysts hybridizes with 1.8% of the nonrepeated portion of the rabbit genome. This indicates that at least the quantitative equivalent of about 60,000 unique base sequences 1000 nucleotides in length is transcribed. RNA from embryos implanted in the uterus (12-day) hybridizes with 2.5% of the nonrepeated DNA. The extent to which these transcripts in early rabbit embryos are unique to this stage of the mammalian life cycle is not known. About 70% of the newly synthesized heterogeneous RNA (hRNA) from 4- and 6-day blastocysts hybridizes with the slow-renaturing or nonrepeated portion of the genome. Since estimates of messenger RNA (mRNA) content of hRNA (Schultz et al., 1973), based on polyadenylic acid content, suggest that about 20% of the hRNA is mRNA, it appears that the majority of the transcripts from nonrepeated DNA in preimplantation rabbit embryos is not mRNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00485738
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