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  • 1970-1974  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 106 (1972), S. 159-167 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytoplasmic disarrangements in the root hairs of Lepidium sativum caused by the vital stain Na-fluorescein (Uranine) after applications of different duration were analyzed by electron microscopy. After an application time of eight min there appear microbody-like structures and vacuoles in the cytoplasm. After a 16-min application severe disorganizations of membranes are brought about. There are distortions and dissolutions of the internal mitochondrial structures. The long cisternae of the ER are fragmented. Vesicle-like structures with a twofold border appear, which probably arise from Golgi cisternae transformed into “rings”. Between the cell wall and the plasmalemma, which is masked by a substance of high contrast, vacuole-like structures are formed. As these processes coincide with the extrusion of the dye from the cytoplasm, which may be observed in the light microscope, a connection between the two phenomena is assumed, the possibility of which is discussed under the aspect of decompartimentation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 71 (1970), S. 443-449 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die durch den Vitalfarbstoff Chrysoidin G/Y verursachten, lichtmikroskopisch nicht mehr wahrnehmbaren Veränderungen in den Oberepidermiszellen der Zwiebelschalen vonAllium cepa werden einer elektronenmikroskopischen Analyse unterzogen. Dabei erweist sich das Kompartimentierungssystem als besonders empfindlich gegenüber der Einwirkung dieses Azofarbstoffes. Es kommt zu einer gesteigerten Entwicklung lomasomenähnlicher Vesikel, zur Deformierung und stellenweisen Auflösung des ER und wahrscheinlich auch zum Austritt der osmiophilen Substanzen aus den Globuli der Leukoplasten. Diese Erscheinungen werden unter dem Aspekt einer Schädigung der Biomembranen durch den Farbstoff diskutiert. Dabei wird speziell eine Depolarisierung der am Membranaufbau beteiligten Strukturiipide durch das einwertige Chrysoidin-Kation in Betracht gezogen.
    Notes: Summary Chrysoidin G/Y used as a dye in vital staining causes cytoplasmic disorganizations a part of which cannot be recognized in the light microscope. After staining epidermal cells from the upper surface of the scaleleaf ofAllium cepa were analyzed by electron microscopy. Above all it is the system of cytoplasmic compartments that proves to be rather sensitive to the influence of the azo dye. There is an increased formation of lomasome-like vesicles; the ER is disarranged and locally dissolved, and probably osmiophilic substances are extruded from the globuli of the leucoplasts. These phenomena are discussed with special reference to damages done to biomembranes by the dye. Especially a depolarization of the structural lipids incorporated in the membranes, effected by the univalent chrysoidin cation, is taken into account.
    Type of Medium: Electronic Resource
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