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Microspectrophotometry of Trypsin-Leishman-stained Human Chromosomes (1973)

LUNDSTEEN, C. ; ERNST, P. ; PHILIP, J.

[s.l.] : Nature Publishing Group

Nature 242 (1973), S. 613-615

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] To measure the number, position, relative size and intensity of bands produced by stains other than Q, and to avoid photographs of the banding patterns, we have produced diagrams from trypsin-treated chromosomes stained with Leishman (TL) using microspectrophotometry (MSP) directly on the specimen. ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/242613a0

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Studies on the mechanism of chromosome banding with trypsin (1974)

Lundsteen, C. ; Kristoffersen, Lene ; Ottosen, F.

Springer

Human genetics 〈Berlin〉 24 (1974), S. 67-69

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ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Description / Table of Contents: Zusammenfassung Menschliche Chromosomen wurden entweder mit Trypsin oder mit spezifisch gehemmtem Trypsin behandelt und nach Leishman gefärbt. Bandenmuster wurden nur nach Trypsin-behandlung gesehen; das kann bedeuten, daß die bindungsspezifische protolytische Aktivität für das Auftreten von Banden entscheidend ist.

Notes: Summary Human chromosomes were treated with either trypsin or specifically inhibited trypsin and stained with Leishman stain. Bands were only obtained after trypsin treatment. This may indicate that binding-specific proteolytic activity was essential for the production of bands.