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SELECTIVE RETENTION AND FILTRATION OF BRAIN NUCLEIC ACIDS IN AGAROSE GELS (1974)

Petrović, S. L. ; Ivanuš, J. J. ; Rakić, L. M.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 22 (1974), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract— Total nucleic acids of rat brain have been separated by agarose gel chromatography at 2 m-NaCl into DNA. transfer RNA plus low molecular weight RNA. and high molecular weight RNA fractions. The DNA fraction contained less than 1 per cent RNA by weight judged by either short-term or long-term labelling with ortho[32P]phosphate. The high molecular weight RNA fraction contained 28 s and 18 s ribosomal RNAs and a heterogeneous population of 20-60 s RNAs, apparent after short-term labelling and characterized by a high content of nearest-neighbour-labelled uridylic acid. The rapidly sedimenting (〉30s) portion of these RNAs could be largely separated from ribosomal RNAs by gel filtration using 4% agarose. The ribosomal RNAs could be fully resolved into 28 s and 18 s components by agarose gel chromatography at 0.5 m-0.6 m-NaCl, as shown by analysis of their sedimentation and nucleotide composition.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1974.tb07595.x

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Natural lymphocytic rosettes in non-operated, thymectomized and bursectomized chickens (1974)

Isaković, K. ; Petrović, S. ; Marković, B. M. ; [et al.]

Springer

Cellular and molecular life sciences 30 (1974), S. 1204-1205

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ISSN: 1420-9071

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Résumé On démontre la présence des lymphocytes capables de faire des rosettes spontanées ou naturelles avec les globules rouges de cobaye, dans le thymus, la bourse de Fabricius, la rate et dans la moelle osseuse des poulets non-immunisés, âgés de 8 semaines. Les effets de la boursectomie et de la thymectomie néonatale chez le poulet, suggèrent l'existence de 2 populations de lymphocytes capables de faire des rosettes, dont l'une thymo-dépendente et l'autre dépendant de la bourse.