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Interaction of proflavine and 9-aminoacridine with DNA at temperatures below and above the melting temperature (1971)

Jordan, D. O. ; Sansom, L. N.

New York : Wiley-Blackwell

Biopolymers 10 (1971), S. 399-410

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ISSN: 0006-3525

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The influence of temperature on the binding of 9-aminoacridine and of proflavine to E. coli DNA in 10-3M NaCl solution has been determined by a spectrophotometric technique. The inadequacy of the expression normally used for the determination of the extent of binding is discussed with reference to measurements at temperatures above which dissociation of the double helix occurs. A method of determining the relative extents of binding to native and denatured DNA at elevated temperatures is described.

Additional Material: 10 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bip.360100214

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