ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Ribonucleic acid from vesicular stomatitis virus (VSV) virions (B particles), defective particles (T), and infected cells was examined by polyacrylamide gel electrophoresis, and apparent molecular weights were estimated. B particle RNA of 4.0×106 daltons from Indiana strain L and from Cocal virus was distinguishable from RNA of 4.5×106 daltons from Indiana strain BT-78 and several strains of New Jersey VSV. Abundant T particle RNA of 1.0×106 daltons, was present in one Indiana L stock and was absent in a plaque purified stock. Cocal virus had T particle RNA of 0.7×106 daltons, and one stock of Ogden strain New Jersey VSV had T particle RNA of 1.2×106 daltons. Little or no T particle RNA was detectable in several other VSV stocks. Major RNA components with apparent molecular weights, 2.4, 0.66, and 0.31×106 daltons in cells infected with Indiana strain L were complementary to virion RNA. The size relationships of B particle RNA from Indiana L and New Jersey strains were examined by sucrose density gradient sedimentation of undenatured and of formaldehyde denatured RNA. Sedimentation of undenatured RNA led to a conflict with gel electrophoresis results, inferring New Jersey had a lower molecular weight than Indiana L, but upon denaturation the relation was consistent with electrophoresis results.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01241543
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