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  • 1970-1974  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Extracellular space in frozen and ethanol substituted central nervous tissueThis investigation was supported by a grant from the National Science Foundation (GB 6698) and from the Barber Fund. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 166 (1970), S. 117-129 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cerebellar and cerebral cortices were frozen under various conditions; within 30 seconds of circulatory arrest, after eight minutes of asphyxiation, after ten minutes of perfusion with glutaraldehyde and after perfusion with this fixative and osmium tetroxide postfixation. Ethanol was used as the solvent in freeze substitution of these tissues. The resulting EMs closely resembled those of similar material freeze substituted in acetone. There were no differences in extracellular space even though differences have been reported between the space in EMs of conventionally fixed material dehydrated with ethanol or acetone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091660109
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Conditions affecting the extracellular space in the frog's forebrainThis investigation was supported in part by a grant from the National Science Foundation (NB 07658). (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 178 (1974), S. 587-597 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The forebrain of the isolated central nervous system of frogs was fixed by freeze substitution and studied with the electron microscope. The extracellular space (ECS) of brains kept for 15-20 min in a physiological salt solution at room temperature varied from an appreciable to a negligible one. In electron micrographs exhibiting a large ECS the tissue elements had a uniform electron density. The EMs with little space featured in some instances a moderate swelling of presynaptic terminals and other tissue elements. Brains kept in a cooled medium or a salt solution with MgCl2 added exhibited invariably an abundant ECS. Treating the brain with a 100 mM KCl solution 5-90 sec before freezing yielded EMs with a contracted ECS and swollen tissue elements, many of which could be identified as dendritic spines. This effect of KCl was in many experiments prevented by bathing the brain in a salt solution containing 10 mM MgCl2 or in a Ca-free solution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091780306
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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