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  • 1965-1969  (3)
  • Cell & Developmental Biology  (3)
  • Chlorophyll
  • Fluorescence quenching
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 126 (1969), S. 255-273 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The duodenal glands of the adult opossum form a lobed, glandular collar in the submucosa immediately distal to the pyloric sphincter. They empty into funnel-shaped mucosal depressions. The depressions, which form two or three irregular rows that run parallel to the pyloroduodenal junction, are lined by either pyloric or intestinal epithelium. An individual gland of Brunner has an extensive intralobular duct system which terminates in a single excretory duct just prior to entry into a depression. Histochemical studies indicate that the duodenal glands elaborate a neutral glycoprotein whereas the duct system appears to produce both neutral and acidic elements. Light and electron microscopic studies have shown the duodenal glands to consist of large, pyramidal cells which lie on a distinct basal lamina. Discrete, mottled or pale secretory granules are found in close association both with the apices of the cells and with the Golgi complexes. The ergastoplasm, cisternae of which are dilated and contain amorphous material, is associated with polysomes and, in the supranuclear region, often becomes markedly dilated, forming what appear to be large vacuoles containing fibrillar material. Blebbing of the ergastoplasm results in the formation of several small vesicles, many of which lie in close association with the Golgi network. Direct membrane continuity has been noted between the two elements.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 126 (1969), S. 275-289 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The postnatal development of Brunner's glands in the opossum has been traced, using 170 specimens divided into 21 groups according to snout-rump length. The newborn opossum has a well developed small intestine, possessing villi and a differentiated intestinal epithelium with concentrations of PAS-positive material in the apical cytoplasm. The ducts of the glands begin to develop within 24 hours after birth and, as they proliferate, two cell types appear. A tall, columnar cell type lines the excretory duct whereas the intralobular duct system is lined by pyramidal cells. Intralobular ducts undergo differentiation by the 50 mm stage and, after this event, secretory units appear as outgrowths from the intralobular duct system. Development continues into early adulthood.Duodenal glands appear to evaginate simultaneously at localized foci, resulting in the formation of a duodenal sinus between glandular elements. The glands evaginate from the mucosa in the most proximal portion of the duodenum, involving both intestinal and gastric mucosal elements. That portion of mucosa lying between the forming glands is submerged and forms the floor of the duodenal sinus. Excretory duct epithelium often lines the sinus in part. The development of Brunner's glands in the opossum is completely independent both of pyloric and intestinal glands.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 70 (1967), S. 141-153 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Exponentially growing L-cells were synchronized by the double thymidine-block method and exposed to high specific activities of tritiated thymidine. DNA, RNA, and protein synthetic rates were measured through one cell cycle with 1-hour pulses of the appropriate C14-labelled precursors. Equivalent doses of tritiated water were substituted for tritiated thymidine in some experiments. Total amounts of DNA and histones per nucleus were determined photometrically in Feulgen and fast-green stained cells.It was observed that incorporated tritiated thymidine has an effect distinct from that of tritiated water and that it enhances the incorporation of the precursors at specific stages of the cell cycle, to a degree roughly proportional to the dose. Photometric data indicated an increase in DNA net synthesis and a metabolic instability of histones in the H3-thymidine-treated cells, resulting in higher DNA:histone ratios.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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