ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Avian reovirus grown in monolayer cultures of chicken kidney cells was partially purified by DEAE-cellulose chromatography and Sephadex column gel filtration. The partially purified virus separated into three or four distinct bands by equilibrium density gradient centrifugation in CsCl. The main band, with a density of 1.381g/ml, was found to contain mainly complete virus particles on electron microscopic examination and contained also infectious particles. The viral RNA extracted with the sodium dodecyl sulfate-phenol method from purified virus, had a complementary base composition,i.e. the ratio of purine to pyrimidine was unity, and the molar per cent of guanine plus cytosine was 42.3%. Thermal denaturation of the RNA in 0.01M Tris-HCl buffer, pH 7.0, containing 5×10−3 M NaCl and 10−4 M EDTA gave a sharp thermal transition and a high melting temperature (92° C). The RNA resisted treatment with formaldehyde and was more resistant to ribonuclease digestion. These results suggested that the RNA of avian reovirus is double stranded.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01242906
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