ISSN:
1432-0878
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A method of fractionation of neuroblasts and spongioblasts perikarya from developing chick embryo cerebral hemispheres is described. The tissue, treated by a mixture of acetone-glycerol-water is dissociated through a nylon sieve. Differential centrifugation on sucrose 1.3 M and 1.8 M has been used to obtain fractions enriched in neuroblasts (80% purity) and spongioblasts (90% purity). The duration of the whole operation is two hours. The evolution and distribution of the cells was studied on dissociated tissue from the 6th day of embryonic life to hatching. The proportion of neuroblasts varies from 13% at 6 days to 36% at 12 days. At the same period the proportion of the sum of indifferent cells and spongioblasts decreases from 81 to 58%, whereas the ependymal cells show little variation (6 to 9%). At hatching these proportions have not changed greatly: neuroblasts, spongioblasts and ependymal cells represent respectively 33, 65 and 2% of the total cells. The cellular differentiation proceeds from the 6th day to hatching. The size of neuroblasts increases and many of them become multipolar. The number of spindle-like cells and small indifferent cells whose origin is probably the germinal layer, decreases together with the thickness of this layer. Early spongioblasts appear only at 10 days and, later on, differentiate into oligodendroblasts and astroblasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00332658
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