ISSN:
0021-9304
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
In the circulation, adherent thrombi are most likely formed by nucleation and growth processes. The adsorption of an anchoring layer must be followed by further constituent interactions; these by crosslinking introduce cooperative effects which affect nucleation and growth rates. Adsorption of thrombin to nonpolar surfaces has been examined using a clotting time assay. Assay discrimination was determined by coefficients of variation: of the assay itself (0.6%), of reproducing diluted thrombin sources (0.37%) and of reproducing sets of dotting tubes containing fibrinogen (1.5%). Adsorptions are 0.54 mg/m2 at the solution-air interface, 0.77 mg/m2 at the solution-clean wall interface, and 0.04 mg/m2 to walls previously treated with thrombin. Average distance between thrombin molecules is 89 Å, the minimum adsorption energy is -11.7 kcal/mole and estimated thrombus adhesive strength 10kg/cm2. Thrombin can be the anchoring layer for a thrombus which contains thrombin, fibrin and platelets since all constituents interact with each other. The thrombin-fibrin intersection energy is about -7 kcal/mole. The platelet can, at least on interaction with thrombin, undergo structural changes which increase interaction. A variety of interactions and cooperative effects are possible. A problem is to develop protocols and assays which will permit specification of the anchoring layer and characteristics of subsequent interactions.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jbm.820030112
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