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  • 1965-1969  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Chloride movements during perfusion fixation with glutaraldehydeThis investigation was supported in part by grant G.B. 6698 from the National Science Foundation. (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 162 (1968), S. 467-477 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cerebral and cerebellar cortices of mice were subjected to a histochemical method for the demonstration of chloride after perfusion fixation with glutaraldehyde. Unfixed, nonasphyxiated cortices subjected to the same chloride method were used as controls. Sections of control material showed a rather uniform chloride distribution in which only the pia and the blood vessels stood out as dark structures containing an appreciable amount of chloride. Sections of glutaraldehyde fixed cerebral cortex showed an accumulation of chloride in apical dendrites. In glutaraldehyde fixed cerebellar cortex the chloride accumulated in the fibers of Bergmann and sometimes in the large dendrites of Purkinje cells. The chloride movement during glutaraldehyde perfusion is in all respects comparable with the movement of chloride caused by asphyxiation of the cerebral and cerebellar cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091620408
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Electron microscopy of the mouse retina prepared by freeze-substitutionThis investigation was supported in part by grant G.B. 4028 from the National Science Foundation. (1968)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 161 (1968), S. 125-139 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The isolated retina of albino mice was fixed by freeze substitution. This method allows the electron microscopic examination of the surface layer of the tissue only. On the scleral side of the retina a considerable space was found between the outer and inner rod segments. In the spaces between the latter, microvilli of the Mueller cells seemed to float freely in the inter-rod material. On the vitreal side extracellular space was observed in bundles of nonmyelinated fibers which are destined to form the optic nerve, and often in the inner plexiform layer. Treating the retina with 5 mM glutamate caused ballooning of elements in the plexiform layer (probably dendrites) and sometimes of the inner and outer rod segments. No obvious swelling was observed of the Mueller cells, the ganglion cells and of the nonmyelinated nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091610113
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Dorsal roots of the rabbit investigated by freeze-substitutionSupported by a grant from The National Science Foundation (GB 2055). (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 152 (1965), S. 283-292 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Dorsal roots from adult rabbits prepared by a freeze-substitution technique and studied by electron microscopy have been compared with those obtained by standard fixation with OsO4. The essential features of myelinated and nonmyelinated fibers and their associated Schwann cells are similar in appearance after both types of fixation. There are, however, slight differences. The extracellular clefts between the nonmyelinated axons and their associated Schwann cells are wider in freeze-substituted roots (200 to 250 Å versus 100 to 150 Å). The major dense lines in the myelin sheath are wider (45 to 55 Å versus about 30 Å) and their radial repeat period is larger (145 to 155 Å versus 100 to 120 Å) in freeze - substituted material than in routinely fixed nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091520308
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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