ISSN:
0022-3832
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The effect of temperature on the equilibrium level of the G-F transformation of actin was investigated in solutions containing very low concentrations of salts. It is shown by physical methods that the G-F transformation is favored by higher temperatures and the F-G transformation by lower temperatures within the range 0-20°. On the basis of the assumption that the transformation is a reversible condensation, the critical actin concentration was obtained was a function of temperature in a given solvent. Analysis of results by a conventional method gives a positive enthalpy change of the order of 10 kcal./mole actin for the G—F transformation. The overall reaction of the G—F transformation is postulated as a transition of hydrated G-actin into dehydrated F-actin plus free water, with net increases in enthalpy and entropy. A chemical method is employed for determining G-actin concentration in equilibrium solutions. Almost all actin other than F-actin filaments must be G-actin (ATP-actin).
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/pol.1960.1204414304
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