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  • 1
    Electronic Resource
    Electronic Resource
    Clustering of null mutations in the EcoRI endonuclease (1987)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 2 (1987), S. 273-282 
    Details
    ISSN: 0887-3585
    Keywords: protein-DNA interactions ; hydroxylamine mutagenesis ; dimerization ; protein structure-function ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: EcoRI endonuclease mutants were isolated in a methylase-deficient background following in vitro hydroxylamine mutagenesis of plasmid pKG2 (Kuhn et al.: Gene 44:253-263, 1986). Mutants which survived high-level endonuclease expression (IPTG induction) were termed null mutants. Sixtytwo of 121 null mutants tested by Western blot contained normal levels of endonuclease cross-reacting protein. The complete endonuclease gene was scquenced for 27 null mutants. This group was found to consist of 20 signle base-change missense mutations, 6 double mutations, and 1 triple mutation. Ten of the 20 signle mutations were clustered between residues 139 and 144. When examined with respect to the structure of the EcoRI-DNA complex (McClarin et al.: Science 234:1526-1541, 1986), these alterations werre found to fall predominantly into two classes: substitutions at the protein-DNA interface or substitutions at the protein-protein (dimer) interface. Protein from several of the mutants was purified and sized by using HPLC. Wild-type EcoRI endonuclease and protein from three of the DNA interface mutations (A1a139→Thr, Gly140→Ser, Arg203→Gln) appeared to be dimeric, while protein from subunit interface mutations (Glu144→Lys, Glu152→Lys, Gly210→Arg) migrated as monomers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prot.340020403
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  • 2
    Electronic Resource
    Electronic Resource
    Über die Oxydationsgeschwindigkeit von Bromwasserstoff mittels Chromsäure in Gegenwart von Salzen (1929)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 177 (1929), S. 137-144 
    Details
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19291770116
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  • 3
    Electronic Resource
    Electronic Resource
    An HPLC procedure for the enantiomeric purity of an optically active cytosine analogue using ligand exchange (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 2 (1990), S. 275-279 
    Details
    ISSN: 0899-0042
    Keywords: nucleotide analogue ; antiviral ; chiral separation ; complexation ; ion-pairing ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An acylonucleotide analogue, 1-[3-hydroxy-2-(phosphonyl-methoxy)propyl]cytosine (HPMPC), has shown activity against herpes simplex Type I and Type II viruses. An HPLC separation of the R- and S-enantiomers of HPMPC by ligand exchange using a mobile phase containing phenylalanine as the chiral modifier and copper(II) as the metal ion is reported.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chir.530020414
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  • 4
    Electronic Resource
    Electronic Resource
    Histologic identification of polyethylene wear debris using Oil Red O stain (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 4 (1993), S. 119-125 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Ultra high molecular weight polyethylene (UHMWPE) wear particles are frequently implicated in causing failure of total joint arthroplasties by eliciting a foreign body reaction. The majority of these particles are subcellular and many are submicron in size. Identification of these small particles of UHMWPE by conventional histologic techniques is difficult. We have therefore investigated the utility of Oil Red O (ORO) stain to identify UHMWPE on histologic sections.A wide variety of specimens was studied including an experimental rabbit model with subcutaneous implantation of polyethylene particles as well as specimens from clinical cases with joint arthroplasties. The sensitivity and specificity of ORO stain was compared to conventional polarized light microscopy for the identification of particulate UHMWPE debris. The ORO stain was found to be as sensitive in identifying particulate UHMWPE debris as polarized light microscopy. However, ORO stain was less specific: two specimens that did not contain any UHMWPE also stained with ORO. Careful examination of standard hematoxylin and eosin stained sections with polarized light was therefore more specific for the identification of particulate UHMWPE. As a single test, the ORO stain does not appear to offer any clear advantage specifically for the identification of UHMWPE. © 1993 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770040202
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid screening of fermentation broths for flavones using tandem mass spectrometry (1993)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1993), S. 84-88 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fermentation broth extracts were rapidly screened for the presence of daidzein and genistein with an approach utilizing the desorption chemical ionization (DCI) and thermospray (TS) ionization modes in conjunction with tandem mass spectrometry (MS/MS). DCI/MS/MS techniques are utilized to rapidly determine the presence of both flavone structures, while TS/liquid chromatographic/MS/MS techniques are utilized for further elucidation and confirmation of the flavone structures. The method is based on the fact that flavone structures undergo similar collisionally activated dissociations (CAD) corresponding to cleavage through the benzopyrone substructure and loss of CO and H2O molecules. A direct comparison, both chromatographic and spectral, to authentic standards is used to confirm the presence of daidzein and genistein.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200220111
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  • 6
    Electronic Resource
    Electronic Resource
    Errata (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 4 (1993), S. 363-363 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770040412
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  • 7
    Electronic Resource
    Electronic Resource
    Polyethylene wear debris and tissue reactions in knee as compared to hip replacement prostheses (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 5 (1994), S. 185-190 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Differences in bearing surface conformity and wear mechanisms suggest that the polyethylene (PE) wear debris generated by total knee replacement (TKR) prostheses should be different than that in total hip replacement prostheses (THR). To address this issue, PE wear debris and the cellular response in periprosthetic tissues from 19 failed TKRs was compared to that from 24 failed THRs using polarized light microscopy and a semiquantitative grading system. The foreign-body inflammatory reaction in the THR cases was characterized by plump macrophages with a diffuse cytoplasmic birefringence when examined under polarized light, indicating the presence of multiple submicron particles of PE. The majority of the PE particles were 〈1 μm in size and only a small fraction of the total were 〉10 μm. The foreign-body inflammatory reaction in the TKR cases was characterized by giant cells with fewer macrophages. In the TKR specimens, the size range of PE particles was broader than in the hips. PE particles between 2 and 20 μm were frequent in TKR specimens; particles 〈1 μm in length were less common than in the THR specimens. Diffuse cytoplasmic birefringence was not a characteristic of the TKR cases. These histologic differences were so consistently distinct that the source of the specimen (i.e., from a THR or TKR) could be blindly determined by light microscopy. The size distribution of the PE wear particles in these cases indicate that THRs generate a higher number of submicron PE particles and relatively few large particles while TKRs generate a broader range of particles that includes fewer submicron particles. The observed differences in the cellular responses is likely a direct result of the differences in the spectrum of PE wear particles. These differences may in part account for differences in periprosthetic bone resorption and loosening in TKRs as compared to THRs. © 1994 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770050302
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  • 8
    Electronic Resource
    Electronic Resource
    The sol/gel contribution to the behavior of γ-irradiated poly(vinylidene fluoride) (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 43 (1991), S. 535-541 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Poly(vinylidene fluoride) films were γ-irradiated in the dose range of 1-20 Mrad, resulting in up to 74% gel. The irradiated polymer undergoes both crosslinking and chain scission, about 5 : 3 events, respectively. Swelling measurements indicate an increasing crosslink density with the gel content, already at the lower doses. Thermal analysis of the gel fraction and the unextracted irradiated samples shows that although crosslinking affects the crystallization, degree of crystallinity, and the melting characteristics, the behavior of the crosslinked material is predominantly controlled by the extractable sol fraction which consists of the more mobile original chains, branched chains, and degraded ones. The crosslinks, already at low density, retard the development of ordinary crystalline polymer morphology.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1991.070430314
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  • 9
    Electronic Resource
    Electronic Resource
    Low dose γ-irradiation of some fluoropolymers: Effect of polymer chemical structure (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 45 (1992), S. 783-795 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The effect of low dose (1-20 Mrad) γ-irradiation on five fluoropolymers (PVF, PVDF, ETFE, FEP, PFA), differing in fluorine content and chain structure, was studied. The radiation effect, reflected by changes in thermal and mechanical properties, as well as NaOH etched surface morphology, was investigated. The main chain structure was found to predominate the irradiated polymers' behavior. The tensile strength of PVF and its resistance to etching increased with radiation dose, while its degree of crystallinity and melting temperature decreased. These changes were correlated with network formation by irradiation and by the incorporation of crosslinked segments into the crystalline phase during recrystallization. Solvent extraction of PVDF (no solvents were found for the other fluoropolymers) revealed increased gel formation with increasing dose. The degree of crystallinity and the tensile strength dependence on radiation dose for PVDF and ETFE reflected the competition between crosslinking and chain scission events. FEP and PFA undergo predominantly chain scission accompanied by reduction of their mechanical properties. PFA even exhibited an increase in both its melting temperature and degree of crystallinity, stemming from a massive degradation process forming shorter chains. Chemical mechanisms are discussed in an attempt to correlate the irradiation effects with the polymers' chain structure.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070450504
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  • 10
    Electronic Resource
    Electronic Resource
    Nonlinear viscoelastic response in two dimensions - numerical modeling and experimental verification (1984)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 24 (1984), S. 1409-1416 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Recently we developed a nonlinear viscoelastic constitutive model which called the Phases Model (PHM) [10]. The main advantages of the PHM over existing nonlinear viscoelastic models are that it lends itself to simple, straightforward calibration of the material functions, and that it is naturally suitable to step-by-step computer simulation. In this paper we apply the PHM to two dimensional (2-D) situations. To this end, we've developed a 2-D finite difference code in cylindrical geometry which is based on the PHM. We demonstrate the validity of our modeling by applying the code to a 2-D set-up developed for this purpose. The 2-D specimen is in the form of a disc of the test material compressed between two rigid metal anvils by a velocity-controlled Instron machine. We monitor the response of the viscoelastic disc in terms of the overall axial force history, and the axial stress component at the disc center. We get good agreement between theory and experiment.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760241807
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