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  • 1
    Electronic Resource
    Electronic Resource
    A modified θ-toxin produced by limited proteolysis and methylation: a probe for the functional study of membrane cholesterol
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 1023 (1990), S. 441-448 
    Details
    ISSN: 0005-2736
    Keywords: (C perfringens) ; Cholesterol heterogeneity ; Cytolysin ; Erythrocyte ; Lymphoma B cell ; θ-Toxin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(90)90137-D
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  • 2
    Electronic Resource
    Electronic Resource
    p-SINE1-like intron of the CatA catalase homologs and phylogenetic relationships among AA-genome Oryza and related species (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 853-861 
    Details
    ISSN: 1432-2242
    Keywords: Key words Catalase ; Oryza ; Rice ; Evolution ; p-SINE1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Intron-2 of the Oryza sativa CatA catalase gene is similar in nucleotide sequence to p-SINE1, a retroposon, and seems to have been added to the ancestral genome of rice. To examine when the p-SINE1-like intron was inserted into CatA during the evolutionary divergence of Oryza species, and to elucidate the evolutionary relationships among Oryza species using the sequence of the intron as a marker, we performed polymerase chain reaction (PCR) analyses of 32 accessions of 17 Oryza species with various genome types. Agarose-gel electrophoresis of the PCR products revealed that all the Oryza species with an AA genome have the CatA homolog with the intron, whereas other Oryza species have the CatA homolog without the intron. These results indicate that intron-2 of CatA is a good marker for distinguishing species with an AA genome among Oryza species. Sequencing of the PCR products showed that all the introns are similar to p-SINE1, though with slight variations in length. We also performed PCR analyses using four accessions of three species in genera related to Oryza, and found that there is an intron in the CatA homolog of Leersia perrieri. On the other hand, the CatA homolog of Porteresia coarctata has no intron. Sequence data showed that the L. perrieri homolog has a p-SINE1-like intron similar to that in Oryza species with an AA genome. These results suggest that the p-SINE1-like intron was already present in the common ancestor of Oryza and L. perrieri and was then lost in the ancestors of P. coarctata and of the Oryza species other than those with an AA genome. The phylogenetic tree of Oryza species with an AA genome based on the nucleotide sequences of the introns leads us to propose that Oryza species with an AA genome evolved from an ancestor of Oryza longistaminata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051144
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  • 3
    Electronic Resource
    Electronic Resource
    Evolutionary relationship of plant catalase genes inferred from exon-intron structures: isozyme divergence after the separation of monocots and dicots (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 9-19 
    Details
    ISSN: 1432-2242
    Keywords: Key words Catalase ; Rice ; Gene structure ; Evolution ; Transposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to understand the molecular evolution of catalase genes in higher plants, we compared the exon-intron structures of 12 genomic sequences from six plant species. It was assumed that the putative single primordial catalase gene had seven introns, because only those catalase genes having this structure are found in the monocotyledonae and dicotyledonae classes. After the evolutionary divergence of monocots from dicots, consecutive duplication of the primordial gene followed by the differential loss of introns occurred in each class to form three (or possibly four in dicots) diverse isozyme genes. In monocots, three ancestral isozyme genes were formed before the divergence of ancestral rice and maize. One of the rice genes, CatA, has an entirely new short intron which was not found in any other plant catalase gene examined. We have investigated the existence of the intron in the CatA homolog in other rice species by polymerase chain reaction (PCR) analysis. One major PCR product was found with the genomic DNAs from O. sativa (indica and japonica types), O. rufipogon and O. glaberrima. DNAs from several accessions of O. longistaminata showed variation in both the number and size of the DNA fragments amplified. PCR analyses and sequencing of the PCR products revealed that there are several CatA homologs having different sequences in some accessions of O. longistaminata. We have extended our study to other species in the Poaceae. The results suggest that the gain of the intron, most likely by insertion of a retroposon, took place in the ancestral genome of rice after its evolutionary divergence from other ancestral cereals such as barley, wheat and oat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050861
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  • 4
    Electronic Resource
    Electronic Resource
    Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 170-176 
    Details
    ISSN: 1432-2242
    Keywords: Key words Saturation mapping ; Yeast artificial chromosome (YAC) ; Rice ; Pi-b ; Blast disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saturation mapping of a very small genomic region is indispensable for map-based cloning. We applied a method based on sub-cloning and the Southern-hybridization technique for generating RFLP markers directly from yeast artificial chromosomes (YACs). Two YACs overlapping each other and covering the locus of the rice blast resistance gene, Pi-b, were used to construct a plasmid sub-library. Rice-specific and single-copy clones suitable as probes for RFLP analysis were selected from this sub-library by hybridization to the blots of digested DNAs of rice, YACs, and yeast. As a result, 22 markers were produced within a small chromosomal region including Pi-b. This case study shows that overlapping YACs known to cover the gene of interest are very useful in fine-scale physical mapping leading to map-based cloning of the target gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050396
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  • 5
    Electronic Resource
    Electronic Resource
    A Tourist element in the 5′-flanking region of the catalase gene CatA reveals evolutionary relationships among Oryza species with various genome types (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 493-500 
    Details
    ISSN: 1617-4623
    Keywords: Key words Catalase ; Oryza ; Rice ; Evolution ; Tourist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tourist-OsaCatA, a transposable element, was found in the 5′-flanking region of the rice gene CatA. The characteristics of this element are similar to those of the other Tourist elements so far found in Oryza sativa. PCR and sequence analyses of 37 accessions of 18 species revealed that all the Oryza species examined, except for one accession, have either a full-length or a partial Tourist element at this locus. Unlike the Tourist elements previously reported, this Tourist element is found in all four Oryza species complexes in the Oryzeae tribe. All AA genome Oryza species, except O. longistaminata, contain the full-length Tourist element. O. longistaminata and the species of the O. officinalis, O. meyeriana and O. ridleyi complexes contain the partial element. A phylogenetic tree of Oryza species based on the nucleotide sequences of these Tourist elements was constructed. The O. longistaminata accessions were placed near the neighboring cluster of the officinalis complex. We propose that the ancestor of O. longistaminata and that of other species with the AA genome diverged, and the ancestor(s) of the O. officinalis, O. ridleyi and O. meyeriana complexes then diverged from the ancestor of O. longistaminata in the course of the evolution of the Oryza species. The Tourist elements associated with CatA and its orthologs thus provide useful tools for examining evolutionary relationships among Oryza species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051110
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  • 6
    Electronic Resource
    Electronic Resource
    Fracture toughness of weldlines in thermoplastic injection molding (1993)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 33 (1993), S. 996-1001 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Weldlines are fatal defects in many injection moldings of thermosetting resins and thermoplastics. Significant strength reduction by weldlines in thermoplastics is caused by poor adhesion, molecular orientation, and a V-notch effect. These factors have been little investigated in detail, in spite of being well known. In the present article, the V-notch effect on strength is discussed for two types of thermoplastics, polystyrene and polycarbonate. The depth of weldlines was obtained by milling on the weldline surface, and the fracture toughness was measured with a double edge notched specimen. Polystryrene, which was drastically weakened by weldlines, had relatively deeper V-notch regions and the fracture toughness was also reduced by weldlines. Although polycarbonate had the same fracture toughness as polystyrene, it had strong weldlines since the depth of weldlines was negligibly small.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760331509
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  • 7
    Electronic Resource
    Electronic Resource
    Gas permeabilities of cellulose nitrate/poly(ethylene glycol) blend membranes (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 2387-2393 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The permeability (P) of cellulose nitrate (CN)/poly(ethylene glycol) (PEG) blend membranes for N2, O2, and CO2 has been measured as a function of film composition. The system CN/PEG-300 showed excellent miscibility, and films of the composition from 100/0 to 50/50 could be used for permeability measurements. P for each gas has been found to be almost constant or rather slightly lowered up to ca. 20 wt % PEG-300 content and then increased appreciably with increasing fraction of PEG. The increment of permeability was most remarkable for CO2, and hence the permselectivity for CO2 was considerably enhanced. Such a behavior of P has been found to be attributable to the plasticizing effect of PEG molecule lowering the glass transition temperature of the blend polymers. The effect of the molecular weight of PEG and that of closed voids generated in glassy blend membranes fabricated from acetone cast on gas permeabilities have been also discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1982.070270708
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