ISSN:
1433-4909
Keywords:
Key wordsCalyptogena soyoae
;
Deep-sea clam
;
Cell culture
;
DNA synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Tissue culture for the deep-sea clam Calyptogena soyoae (C. soyoae) has been examined. Mantle tissue was cultured in Dulbecco's modified Eagle medium that was prepared using artificial seawater supplemented with fetal bovine serum (FBS) and the body fluid of C. soyoae. The mantle cells were viable in culture for at least 13 days at 4°C and atmospheric pressure on a polylysine-coated dish, although no cells attached in the body fluid-free culture medium. It was found that mantle cells synthesized DNA and seemed to proliferate under atmospheric conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007920070009
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