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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 733 (1983), S. 201-209 
    ISSN: 0005-2736
    Keywords: (Phospholipid membrane) ; Infrared spectroscopy ; Linear dichroism ; Melittin orientation ; Membrane-protein interaction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of psychiatry and clinical neuroscience 220 (1975), S. 361-371 
    ISSN: 1433-8491
    Keywords: Early Brain Damage ; Psychic Diseases ; Course of the Disease ; AMP-System ; Frühkindlicher Hirnschaden ; Psychische Erkrankungen ; Verlauf-charakteristika ; AMP-System
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Daten von 1926 stationär behandelten, psychiatrischen Patienten der Jahre 1968–1972, welche mit dem AMP-Dokumentationssystem erfaßt worden waren, wurden hinsichtlich der Häufigkeit des anamnestischen Merkmals „Frühkindlicher Hirnschaden“ ausgewertet. Es fanden sich 82 Merkmalsträger (4,26%). Zu jedem Patienten mit frühkindlichem Hirnschaden wurde ein Kontrollfall gleichen Alters, Geschlechts und gleicher Diagnosengruppe extrahiert. Die Patientengruppe mit einer Minderbegabung wurde gesondert untersucht. Auf Grund methodischer Schwierigkeiten war damit zu rechnen, daß die gefundene Häufigkeit eine Unterschätzung darstellt und daß sich feinere Unterschiede zwischen Index- und Kontrollgruppen nicht nachweisen lassen, daß die gefundenen Unterschiede jedoch real sein dürften. Die frühkindlichen Hirnschäden fanden sich am häufigsten in der Gruppe „andere Diagnosen“, die unter anderem die psychischen Störungen bei der Epilepsie enthielt. Bei Neurosen und Schizophrenien fand sich das Merkmal etwa gleich häufig, und zwar der gefundenen Durchschnittshäufigkeit der psychiatrischen Population entsprechend. Signifikant unterrepräsentiert war es bei den affektiven Psychosen und involutiven Erkrankungen. Durch die frühkindliche Hirnschädigung kam es zu einer deutlichen Vorverlegung des Manifestationsalters, die psychische Erkrankung zeigte einen mehr chronischen Verlauf. Es wurde versucht, die ungleichmäßige Verteilung der frühkindlichen Hirnschäden über das diagnostische Spektrum dadurch zu erklären, daß hypoxidotische perinatale Schäden auch keine diffusen, unspezifischen Störungen hervorrufen, sondern vorwiegend zu sensorischen Behinderungen und möglicherweise zu einer Störung im aminergen System führen. Es wurden weiterhin frühkindliche Hirnschäden als ein Modus der Transmission psychischer Erkrankungen diskutiert.
    Notes: Summary The data of 1926 psychiatric in-patients documented by the AMP-system were evaluated with respect to the anamnestic item “early brain damage”. There were 82 cases (4.26%). For each patient with an early brain damage a control-case matched for age, sex and diagnosis was selected. Patients with mental handicaps were examined seperately. For methodical reasons the demonstrated frequencies are an underestimation, and subtle differences between index- and controlgroups are not expected to be visible, but those differences which could be found, will be real. The highest frequency for “early brain damage” was found in the diagnostic group which also contained the psychic disturbances in epilepsy. Among neuroses and schizophrenia the item was found with the average-frequency of the total psychiatric population. It was under-represented among affective psychoses and involutional diseases. An early brain damage predisposed to an earlier age of first manifestation, and the psychic disease showed a more chronic course. Hypotheses concerning the uneven distribution over the diagnostic spectrum were discussed with particular emphasis on the fact that hypoxic perinatal disturbances do not lead to diffuse and unspecific consequences, but predominantly to a sensory impairment and possibly to a deficit in the aminergic system. Early brain damage was also discussed as one mode of the transmission of psychic diseases.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0778
    Keywords: 5-HT3 receptor ; large scale ; reactor ; Semliki Forest virus ; suspension process ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The expression of recombinant proteins with the Semliki Forest Virus (SFV) system has been scaled up to bioreactor scale. As a model protein for this study the human 5-HT3 receptor was chosen. The gene for the receptor was subcloned into the SFV expression plasmid pSFV1. Virus production by in vivo packaging and production of the recombinant protein was scaled up, the latter to a reactor volume of 11.5 l. A VibromixTM agitation system was chosen to overcome aggregation problems of BHK cells in suspension. In the process, cells were first grown to a density of 106 cells/ml, the medium was then exchanged with fresh medium and the culture was infected with the recombinant virus at an estimated multiplicity of infection of 30. 24 h post infection we measured an expression level of 3 million functional 5-HT3 receptors per cell. For harvesting, the cells were pelleted by centrifugation. The receptor protein was purified in a single step (Hovius et al., 1998) by exploiting the hexa-His tag at minimal protein loss (51% yield). Experiments to optimise expression resulted in yields up to 8 million receptors per cell, when the pH of a suspension culture was controlled at pH 7.3. Rapid virus generation and protein production, high protein yields as well as successful large scale application have made the SFV expression system attractive to produce large quantities of recombinant protein in a very short time. After optimisation of the expression conditions (in particular by setting the pH at 7.3), yields were increased twofold.
    Type of Medium: Electronic Resource
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