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Two-dimensional DNA gel electrophoresis as a method for analysis of eukaryotic genome structure: Evaluation using Tetrahymena thermophila DNA

Huvos, P.E. ; Sanchez, J.A. ; Kramer, K.M. ; [et al.]

Amsterdam : Elsevier

Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 949 (1988), S. 325-333

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ISSN: 0167-4781

Keywords: (Protozoa) ; DNA methylation ; DNA rearrangement ; Electrophoresis ; Hybridization ; Repeated DNA sequence ; Two dimensional DNA gel electrophoresis

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology , Chemistry and Pharmacology , Medicine , Physics

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(88)90159-5

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Combined Assessment of Genetic Variability in Populations of Brown Trout (Salmo trutta L.) Based on Allozymes, Microsatellites, and RAPD Markers (1999)

Cagigas, M.E. ; Vazquez, E. ; Blanco, G. ; [et al.]

Springer

Marine biotechnology 1 (1999), S. 286-296

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ISSN: 1436-2236

Keywords: Key words:Salmo trutta L., molecular markers, allozyme microsatellite, randomly amplified polymorphic DNA (RAPD)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract: Genetic variability within and among four Spanish natural populations of Salmo trutta L. was evaluated on the basis of 25 enzyme loci, 3 microsatellite loci, and 9 randomly amplified polymorphic DNAs (RAPDs). A total of 21 allelic markers were found, 12 of which were reported by microsatellites, whereas enzyme and RAPD accounted only for 6 and 3, respectively. Genetic variation within samples was significantly higher for microsatellites and RAPD than for enzyme loci. Although all methods reported a high degree of allelic heterogeneity among samples, also revealing a high degree of gene diversity, genetic relationships depicted by UPGMA dendrograms closely agreed for all kinds of data. Microsatellite loci appeared to be the most feasible technique when searching for specific alleles for a population or an area, owing to the higher number of allelic variants found.