ISSN:
1573-4994
Schlagwort(e):
4′, 6-Diamidino-2-phenylindole (DAPI)
;
pepsin, fluorescence, circular dichroism, binding
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Physik
Notizen:
Abstract We recently found that the fluorescent dye DAPI, well-known for its use with nucleic acids, is also able to interact with proteins as well as ordered phospholipids assemblies. The interaction of DAPI with pepsin under different conditions of pH and ionic strength was studied with fluorescence and circular dichroism techniques. From a comparison of the results obtained, the interaction appears to be rather tight and specific, dependent on both electrostatic and hydrophobic forces, and able to probe the tridimensional conformation of the protein.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00865267
Permalink