ISSN:
1432-072X
Keywords:
(p)ppGpp
;
α-Methylglucoside
;
Pseudomonic acid
;
Granaticin
;
2,4-Dinitrophenol
;
RNA synthesis
;
GDP-, GTP-pool
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The response of the thermophile Bacillus stearothermophilus to inhibition of tRNA acylation, energy starvation and temperature downshift was characterized. We found that B. stearothermophilus, like other prokaryotic organisms, reacts with the so-called stringent response, which includes the accumulation of the unusual nucleotides guanosine 3′,5′ bis (dipphosphate) [ppGpp] and guanosine 3′-diphosphate, 5′-triphosphate [pppGpp] and concomitantly the reduction of RNA synthesis and growth rate. The amount of (p)ppGpp formed depended on the cause of the stringent response: when tRNA acylation was inhibited (p)ppGpp synthesis was much higher than after energy starvation or temperature downshift whereas RNA synthesis was totally blocked in each case.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00417174
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