ISSN:
1573-6903
Keywords:
Serotonin
;
neuronal serotonin transporter
;
calf striatum
;
[3H]imipramine binding
;
[3H]paroxetine binding
;
purification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The serotonin (5-HT) transporter from calf striatum cerebral membranes was solubilized with digitonin and characterized by gel exclusion chromatography. [3H]Imipramine and [3H]paroxetine were utilized as markers for labeling it.3H-imipramine labels a high- and a low-affinity site on striaturn membranes, whereas it binds to a single high-affinity site on the solubilized fraction. [3H]Paroxetine binds with the same affinity to a single site on both membranes and solubilized preparations. After gel exclusion chromatography of the solubilizate both [3H]imipramine and [3H]paroxetine bind on an identical fraction of 205 kDa molecular weight, with a similar maximum number of binding sites (Bmax). Our results suggest that both3H-imipramine and [3H]paroxetine bind to a common site on the 5-HT transporter.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01006821
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