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  • 1
    Electronic Resource
    Electronic Resource
    Solubilization and partial purification of GABA"B receptor from bovine brain
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 172 (1990), S. 22-27 
    Details
    ISSN: 0006-291X
    Keywords: [abr] CHAPS; 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate ; [abr] GABA; γ -aminobutyric acid
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(05)80167-6
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Solubilization and partial purification of GABA"B receptor from bovine brain
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 172 (1990), S. 22-27 
    Details
    ISSN: 0006-291X
    Keywords: [abr] CHAPS; 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate ; [abr] GABA; γ -aminobutyric acid
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(05)80167-6
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Immunoprecipitation of 125I-insulin crosslinked receptor (1983)
    Springer
    Diabetologia 24 (1983), S. 387-390 
    Details
    ISSN: 1432-0428
    Keywords: Crosslinking ; disuccinimidyl suberate ; insulin receptor ; anti-receptor antibody ; immunoprecipitation ; type B syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 125I-insulin was covalently crosslinked to its receptor on human placental membrane fractions with disuccinimidyl suberate. The 125I-insulin crosslinked receptor was solubilized with Triton X-100 and used as a probe to determine autoantibodies to the insulin receptor in sera from patients with insulin resistance (type B syndrome). When the solubilized 125I-insulin: receptor complex was incubated with these sera and then with anti-human IgG serum, the complex was precipitated as a function of the amount of anti-receptor serum. Unlabelled insulin at concentrations ⩽10 μg/ml did not affect immunoprecipitation of the complex, suggesting that a subpopulation of anti-receptor antibodies recognizes determinants outside the insulin binding region of the receptor molecule. 125I-insulin: receptor complex was precipitated also by the addition of anti-insulin antibodies. The effect of anti-insulin antibody was eliminated by the addition of excess unlabelled insulin. The immunoprecipitation assay using 125I-insulin crosslinked receptor was sensitive and convenient for detecting anti-receptor antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251830
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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