ISSN:
1432-072X
Keywords:
Corynebacterium glutamicum
;
Lactose utilization
;
β-galactosidase
;
Lactose permease
;
Galactose accumulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The entire Escherichia coli lactose operon was inserted into an E. coli/Corynebacterium glutamicum shuttle vector and introduced into the gram-positive host organism C. glutamicum R163. Recombinant C. glutamicum strains carrying the lac genes downstream of an efficient promoter displayed rapid growth with lactose as the sole source of carbon. Two prerequisites were necessary to obtain good growth of C. glutamicum R 163 on lactose: presence of the lacY gene in addition to lacZ and an appropriatc promoter for efficient transcription in C. glutamicum. The galactose moiety of lactose was not utilized but accumulated in the culture broth. C. glutamicum strains carrying only the lacZ (β-galactosidase) gene but not lacY (lactose permease) were not able to grow in lactose minimal medium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245357
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