ISSN:
1573-3904
Keywords:
aggregation
;
assignment
;
peptide T
;
β-sheet
;
symmetry
;
transferred NOE
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract There is currently great interest in the study of peptide aggregation by β-sheet formation because of its relevance in pathological states or in the design of self-assembling systems of technological interest. NMR studies of β-sheet aggregates are difficult because of their long correlation times and spectral degeneracy. In this communication we demonstrate the combination of a semiselective TOCSY-NOESY experiment with partial deuterium exchange of labile protons to assign inter-molecular NOE cross peaks and prove the presence of a soluble parallel β-sheet in fast exchange with monomeric Ac-ASTTTNYT-NH2 (Ac-T-NH2) in solution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008968104503
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