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  • 1
    ISSN: 1432-2072
    Keywords: Dopamine agonists ; Autoreceptors ; 3-PPP ; Neuroleptics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pharmacological profile of the enantiomers of the proposed selective dopamine (DA) autoreceptor agonist 3-PPP [3-(3-hydroxyphenyl)-N-n-propylpiperidine] has been studied. In vitro both enantiomers showed weak DA agonistic activity, and (-)-3-PPP some DA antagonistic effect on DA-stimulated adenylate cyclase activity. Both enantiomers in low doses had a similar profile in vivo: Inhibition of locomotor activity of mice and rats, induction of contralateral circling behaviour in 6-hydroxy-DA-lesioned rats and an emetic effect in dogs. At higher doses, differential effects of the enantiomers were found: (+)-3-PPP induced hyperactivity, weak stereotyped behaviour and ipsilateral circling in hemitransected rats. (-)-3-PPP had depressant effects in high doses, inhibited d-amphetamine-induced hyperactivity and d-amphetamine-, methylphenidate- and apomorphine-induced stereotyped licking/biting in rats and antagonized apomorphine-induced emesis in dogs. However, (+)-3-PPP also showed a weak antagonistic activity against d-amphetamine-induced hyperactivity and d-amphetamine-and apomorphine-induced stereotypy in rats and inhibited apomorphine-induced emesis in dogs. It is suggested that both enantiomers have significant effects on postsynaptic DA receptors in high doses: (-)-3-PPP with weak antagonistic activity in some test models and (+)-3-PPP with agonistic and antagonistic effect. Since these effects of (+)-3-PPP were of low intensity at high doses, (+)-3-PPP may be a partial DA agonist at postsynaptic receptors in high doses. Interaction experiments with neuroleptics indicated that only (-)-3-PPP significantly increased the antistereotypic effect of neuroleptics in rats. Therefore, the proposed DA autoreceptor stimulation is possibly unrelated to the neuroleptic potentiation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0630
    Keywords: 89.60 ; 35.80 ; 06.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Laser heterodyne spectroscopy (LHS) techniques with semiconductor laser local oscillators (LO) in the 3–30 μm range have the potential to measure radical gas species in the stratosphere. The goal of this experiment is to measure radical gases from Spacelab, including ClO, ClONO2, HO2, H2O2, N2O5, and HOCl in solar occulation with vertical resolution ≦2km and vertical range from 10 to 70 km. Sensitivity analyses have been performed on ClO and O3 to determine design criteria for the LHS instrument. Results show that O3 and ClO vertical profiles can be measured with an accuracy ≧95% and ≧80%, respectively, over the total profile. These accuracies require the LO to maintain the following characteristics: frequency stability (Δf w≦20 MHz), single-mode power (P LO≧500 μW), and minimum frequency drift (≦5 MHz). Laboratory heterodyne measurements performed with semiconductor lasers generated the same shot-noise photocurrent as CO2 lasers, for comparable single-mode power. “Excess-noise” regions were identified, but could be wavelength controlled by fine control of operating temperature and injection current. Doppler-shift effects and limited solar occultation measurement times due to Spacelab orbits should pose minimum mission constraints on the experiment.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Basic research in cardiology 90 (1995), S. 323-331 
    ISSN: 1435-1803
    Keywords: Na+ ; K+-ATPase ; Ca2+-ATPase ; rat ventricular myocardium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Assays for complete quantification of Na+, K+-and Ca2+-ATPase in crude homogenates of rat ventricular myocardium by determination of K+-and Ca2+-dependentp-nitrophenyl phosphatase (pNPPase) activities were evaluated and optimized. Using these assays the total K+-and Ca2+-dependentpNPPase activities in ventricular myocardium of 11–12 week-old rats were found to be 2.98±0.10 and 0.29±0.02 μmol×min−1×g−1 wet wt. (mean±SEM) (n=5), respectively. Coefficient of variance of interindividual determinations was 7 and 12%, respectively. The total Na+, K+-and Ca2+-ATPase concentrations were estimated to 2 and 10 nmol×g−1 wet wt., respectively. Evaluation of a putative developmental variation revealed a biphasic age-related change in the rat myocardial Ca2+-dependentpNPPase activity with an increase from birth to around the third week of life followed by a decrease. By contrast, the K+-dependentpNPPase activity of the rat myocardium showed a decrease from birth to adulthood. It was excluded that the changes were simple out-come of variations in water and protein content of myocardium. Expressed per heart, the K+-and Ca2+-dependentpNPPase activity gradually increased to a plateau. The present assay for Na+, K+-ATPase quantification has the advantage over [3H] ouabain binding of being applicable on the ouabain-resistant rat myocardium, and is more simple and rapid than measurements of K+-dependent 3-0-methylfluorescein phosphatase (3-0-MFPase) in crude tissue homogenates. Furthermore, with few modifications thepNPPase assay allows quantification of Ca2+-ATPase on crude myocardial homogenates. Age-dependent changes in K+-and Ca2+-dependentpNPPase activities are of developmental interest and indicate the importance of close age match in studies of quantitative aspects of Na+, K+-and Ca2+-ATPase in excitable tissues.
    Type of Medium: Electronic Resource
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