Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-0630
    Keywords: 89.60 ; 35.80 ; 06.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Laser heterodyne spectroscopy (LHS) techniques with semiconductor laser local oscillators (LO) in the 3–30 μm range have the potential to measure radical gas species in the stratosphere. The goal of this experiment is to measure radical gases from Spacelab, including ClO, ClONO2, HO2, H2O2, N2O5, and HOCl in solar occulation with vertical resolution ≦2km and vertical range from 10 to 70 km. Sensitivity analyses have been performed on ClO and O3 to determine design criteria for the LHS instrument. Results show that O3 and ClO vertical profiles can be measured with an accuracy ≧95% and ≧80%, respectively, over the total profile. These accuracies require the LO to maintain the following characteristics: frequency stability (Δf w≦20 MHz), single-mode power (P LO≧500 μW), and minimum frequency drift (≦5 MHz). Laboratory heterodyne measurements performed with semiconductor lasers generated the same shot-noise photocurrent as CO2 lasers, for comparable single-mode power. “Excess-noise” regions were identified, but could be wavelength controlled by fine control of operating temperature and injection current. Doppler-shift effects and limited solar occultation measurement times due to Spacelab orbits should pose minimum mission constraints on the experiment.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1615-6102
    Keywords: Nickel ; Endocytosis ; Motility ; Proliferation ; Cellular nickel content ; Adaptation ; Tetrahymena pyriformis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary At concentrations above 1 mM, nickel has a dose-dependent effect on the rate of food vacuole formation in cells in the growth medium, proteose peptone (PP); total inhibition of endocytosis occurs within 10 minutes in 6mM nickel. However, only a 10 times lower concentration of nickel is tolerated by starved cells in an inorganic salt medium, a difference which may be ascribed to the high binding property of nickel to organic material. In the PP medium, nickel affects cell motility by increasing the rate of movement at a concentration of 1 mM, and by causing immobilization after 30 minutes in 6mM nickel; a spontaneous, partial recovery of cell motility is seen after 3 hours in 6 mM nickel. The effects of nickel on endocytosis and cell motility are reversible after removal of nickel. Cell proliferation continues at a reduced rate in 1 mM nickel, while only 1 1/2 cell doublings are achieved in 3 mM nickel during a 72-hour exposure, and no proliferation occurs in 6mM nickel, where an increasing cell mortality is observed after 12 hours. The cell content of nickel relates initially to the external concentration of the metal; however, cells in 1 mM nickel are capable of maintaining a constant content of the metal, whereas in 3 mM, the rate of accumulation is reduced after 3 hours, and cells in 6mM nickel accumulate the metal at a constant rate. All nickel-treated cells contain small refractive granules, previously proposed as representing an ion-regulating system, and the apparent adaption ofTetrahymena to the effects of nickel may be ascribed to such a regulation of the intracellular concentration of the metal.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Basic research in cardiology 90 (1995), S. 323-331 
    ISSN: 1435-1803
    Keywords: Na+ ; K+-ATPase ; Ca2+-ATPase ; rat ventricular myocardium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Assays for complete quantification of Na+, K+-and Ca2+-ATPase in crude homogenates of rat ventricular myocardium by determination of K+-and Ca2+-dependentp-nitrophenyl phosphatase (pNPPase) activities were evaluated and optimized. Using these assays the total K+-and Ca2+-dependentpNPPase activities in ventricular myocardium of 11–12 week-old rats were found to be 2.98±0.10 and 0.29±0.02 μmol×min−1×g−1 wet wt. (mean±SEM) (n=5), respectively. Coefficient of variance of interindividual determinations was 7 and 12%, respectively. The total Na+, K+-and Ca2+-ATPase concentrations were estimated to 2 and 10 nmol×g−1 wet wt., respectively. Evaluation of a putative developmental variation revealed a biphasic age-related change in the rat myocardial Ca2+-dependentpNPPase activity with an increase from birth to around the third week of life followed by a decrease. By contrast, the K+-dependentpNPPase activity of the rat myocardium showed a decrease from birth to adulthood. It was excluded that the changes were simple out-come of variations in water and protein content of myocardium. Expressed per heart, the K+-and Ca2+-dependentpNPPase activity gradually increased to a plateau. The present assay for Na+, K+-ATPase quantification has the advantage over [3H] ouabain binding of being applicable on the ouabain-resistant rat myocardium, and is more simple and rapid than measurements of K+-dependent 3-0-methylfluorescein phosphatase (3-0-MFPase) in crude tissue homogenates. Furthermore, with few modifications thepNPPase assay allows quantification of Ca2+-ATPase on crude myocardial homogenates. Age-dependent changes in K+-and Ca2+-dependentpNPPase activities are of developmental interest and indicate the importance of close age match in studies of quantitative aspects of Na+, K+-and Ca2+-ATPase in excitable tissues.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...