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  • Neuronal volume  (2)
  • 06.70  (1)
  • Ca2+-ATPase  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The volume of Purkinje cells decreases in the cerebellum of acrylamide – intoxicated rats, but no cells are lost (1994)
    Springer
    Acta neuropathologica 88 (1994), S. 307-312 
    Details
    ISSN: 1432-0533
    Keywords: Key words Acrylamide ; Cerebellum ; Neuronal volume ; Neuronal number ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of acrylamide intoxication on the numbers of granule and Purkinje cells and the volume of Purkinje cell perikarya have been evaluated with stereological methods. The analysis was carried out in the cerebella of rats that had received a dose of 33.3 mg/kg acrylamide, twice a week, for 7.5 weeks. The total numbers of cerebellar granule and Purkinje cells were estimated using the optical fractionator and the mean volume of the Purkinje cell perikarya was estimated with the vertical rotator technique. The volumes of the molecular layer, the granular cell layer and the white matter were estimated using the Cavalieri principle. The mean weight of the cerebellum of the intoxicated rats was 7   % lower than that of the controls rats (2P = 0.001). The numbers of the Purkinje cells and granule cells were the same in both groups, but the mean volume of the perikarya of the Purkinje cells in the intoxicated rats was 10.5   % less than that of the control group (2P = 0.004). The volume of the granular cell layer was reduced by 15   % (2P = 0.006) but there were no differences in the volumes of the molecular layer and the white matter in the intoxicated and control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310374
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Structural preservation of cerebellar granule cells following neurointoxication with methyl mercury: a stereological study of the rat cerebellum (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 251-256 
    Details
    ISSN: 1432-0533
    Keywords: Key words Cerebellum ; Neuronal volume ; Methyl ; mercury ; Neuronal number ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Methyl mercury intoxication causes ataxia. Structural changes of cerebellar and peripheral nerve tissues have been described. However, it is still unclear whether the ataxia is of cerebellar or peripheral origin. To clarify this question further, the effects of methyl mercury intoxication on the numbers of granule and Purkinje cells and the volume of Purkinje cell perikarya have been evaluated with stereological methods. Rats were intoxicated with methyl mercury, at a dose of 2 mg/kg per day for 19 successive days, and the analysis was carried out 2.5 or 4.5 weeks later. The total numbers of cerebellar granule cells and Purkinje cells were estimated using an optical fractionator and the mean volume of the Purkinje cells was estimated by the vertical rotator technique. The volumes of the granular cell layer, the molecular layer and the white matter were estimated using the Cavalieri principle. The intoxicated animals developed hindlimb incoordination when held by the tail. Although pronounced axonal degeneration occurred in the peripheral nervous system, no changes were found in cerebellar cell numbers or cell sizes in either of the test groups. The absence of detectable light microscopic changes in the cerebellum indicates that the peripheral nervous system is affected prior to the cerebellum in rats intoxicated with organic mercury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296508
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  • 3
    Electronic Resource
    Electronic Resource
    An experiment concept to measure stratospheric trace constituents by laser heterodyne spectroscopy (1980)
    Springer
    Applied physics 23 (1980), S. 47-56 
    Details
    ISSN: 1432-0630
    Keywords: 89.60 ; 35.80 ; 06.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Laser heterodyne spectroscopy (LHS) techniques with semiconductor laser local oscillators (LO) in the 3–30 μm range have the potential to measure radical gas species in the stratosphere. The goal of this experiment is to measure radical gases from Spacelab, including ClO, ClONO2, HO2, H2O2, N2O5, and HOCl in solar occulation with vertical resolution ≦2km and vertical range from 10 to 70 km. Sensitivity analyses have been performed on ClO and O3 to determine design criteria for the LHS instrument. Results show that O3 and ClO vertical profiles can be measured with an accuracy ≧95% and ≧80%, respectively, over the total profile. These accuracies require the LO to maintain the following characteristics: frequency stability (Δf w≦20 MHz), single-mode power (P LO≧500 μW), and minimum frequency drift (≦5 MHz). Laboratory heterodyne measurements performed with semiconductor lasers generated the same shot-noise photocurrent as CO2 lasers, for comparable single-mode power. “Excess-noise” regions were identified, but could be wavelength controlled by fine control of operating temperature and injection current. Doppler-shift effects and limited solar occultation measurement times due to Spacelab orbits should pose minimum mission constraints on the experiment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00899570
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Quantification in crude homogenates of rat myocardial Na+, K+-and Ca2+-ATPase by K+ and Ca2+-dependent pNPPase. Age-dependent changes (1995)
    Springer
    Basic research in cardiology 90 (1995), S. 323-331 
    Details
    ISSN: 1435-1803
    Keywords: Na+ ; K+-ATPase ; Ca2+-ATPase ; rat ventricular myocardium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Assays for complete quantification of Na+, K+-and Ca2+-ATPase in crude homogenates of rat ventricular myocardium by determination of K+-and Ca2+-dependentp-nitrophenyl phosphatase (pNPPase) activities were evaluated and optimized. Using these assays the total K+-and Ca2+-dependentpNPPase activities in ventricular myocardium of 11–12 week-old rats were found to be 2.98±0.10 and 0.29±0.02 μmol×min−1×g−1 wet wt. (mean±SEM) (n=5), respectively. Coefficient of variance of interindividual determinations was 7 and 12%, respectively. The total Na+, K+-and Ca2+-ATPase concentrations were estimated to 2 and 10 nmol×g−1 wet wt., respectively. Evaluation of a putative developmental variation revealed a biphasic age-related change in the rat myocardial Ca2+-dependentpNPPase activity with an increase from birth to around the third week of life followed by a decrease. By contrast, the K+-dependentpNPPase activity of the rat myocardium showed a decrease from birth to adulthood. It was excluded that the changes were simple out-come of variations in water and protein content of myocardium. Expressed per heart, the K+-and Ca2+-dependentpNPPase activity gradually increased to a plateau. The present assay for Na+, K+-ATPase quantification has the advantage over [3H] ouabain binding of being applicable on the ouabain-resistant rat myocardium, and is more simple and rapid than measurements of K+-dependent 3-0-methylfluorescein phosphatase (3-0-MFPase) in crude tissue homogenates. Furthermore, with few modifications thepNPPase assay allows quantification of Ca2+-ATPase on crude myocardial homogenates. Age-dependent changes in K+-and Ca2+-dependentpNPPase activities are of developmental interest and indicate the importance of close age match in studies of quantitative aspects of Na+, K+-and Ca2+-ATPase in excitable tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00797910
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    Paper (German National Licenses)
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