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  • Cell elongation  (3)
  • 07.60  (2)
  • Endoplasmic reticulum  (2)
  • Engineering General  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Further studies on the mechanism of inhibition of intestinal chylomicron transport by Pluronic L-81
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1004 (1989), S. 357-362 
    Details
    ISSN: 0005-2760
    Keywords: Chylomicron formation ; Endoplasmic reticulum ; Golgi apparatus ; Intestinal lipoprotein ; VLDL
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0005-2760(89)90084-2
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  • 2
    Electronic Resource
    Electronic Resource
    The role of the epidermis in auxin-induced and fusicoccin-induced elongation of Pisum sativum stem segments (1980)
    Springer
    Planta 150 (1980), S. 371-379 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Epidermis peeling ; Fusicoccin ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of peeling and wounding on the indole-3-acetic acid (IAA) and fusicoccin (FC) growth response of etiolated Pisum sativum L. cv. Alaska stem tissue were examined. Over a 5 h growth period, peeling was found to virtually eliminate the IAA response, but about 30% of the FC response remained. In contrast, unpeeled segments wounded with six vertical slits exhibited significant responses to both IAA and FC, indicating that peeling does not act by damaging the tissue. Microscopy showed that the epidermis was removed intact and that the underlying tissue was essentially undamaged. Neither the addition of 2% sucrose to the incubation medium nor the use of a range of IAA concentrations down to 10-8 M restored IAA-induced growth in peeled segments, suggesting that lack of osmotic solutes and supra-optimal uptake of IAA were not important factors over this time period. It is concluded that, although the possibility remains that peeling merely allows leakage of hydrogen ions into the medium, it seems more likely that peeling off the epidermis removes the auxin responsive tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390172
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of cytochalasin B on the rate of growth and ultrastructure of wheat coleoptiles and maize roots (1979)
    Springer
    Planta 144 (1979), S. 373-383 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cell elongation ; Cytochalasin B ; Dictyosomes ; Secretion ; Triticum ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytochalasin B (CB) inhibits the elongation growth of maize roots, and that of wheat coleoptile segments incubated in indolyl-3-acetic acid, by over 30% after a lag period of about 60 min. This long lag is not due to poor tissue penetration by the inhibitor, but seems to reflect a property of the process inhibited by CB. The only visible ultrastructural change accompanying growth inhibition is the accumulation of secretory vesicles in the vicinity of dictyosomes, which occurs between 90 and 300 min. However, a massive accumulation of vesicles is seen after 120 min in root cap cells which possess very active dictyosomes. The results indicate that CB does not inhibit elongation growth by interfering with cytoplasmic streaming. Instead, they indicate that the drug acts to inhibit the secretion of cell wall components at some stage after vesicle production, but prior to their transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391581
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  • 4
    Electronic Resource
    Electronic Resource
    Laser phase and frequency stabilization using an optical resonator (1983)
    Springer
    Applied physics 31 (1983), S. 97-105 
    Details
    ISSN: 1432-0649
    Keywords: 06 ; 07.60 ; 07.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We describe a new and highly effective optical frequency discriminator and laser stabilization system based on signals reflected from a stable Fabry-Perot reference interferometer. High sensitivity for detection of resonance information is achieved by optical heterodyne detection with sidebands produced by rf phase modulation. Physical, optical, and electronic aspects of this discriminator/laser frequency stabilization system are considered in detail. We show that a high-speed domain exists in which the system responds to the phase (rather than frequency) change of the laser; thus with suitable design the servo loop bandwidth is not limited by the cavity response time. We report diagnostic experiments in which a dye laser and gas laser were independently locked to one stable cavity. Because of the precautions employed, the observed sub-100 Hz beat line width shows that the lasers were this stable. Applications of this system of laser stabilization include precision laser spectroscopy and interferometric gravity-wave detectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702605
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  • 5
    Electronic Resource
    Electronic Resource
    Dye-laser frequency stabilization using optical resonators (1984)
    Springer
    Applied physics 33 (1984), S. 179-185 
    Details
    ISSN: 1432-0649
    Keywords: 06 ; 07.60 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We describe a study, performed using heterodyne techniques, of the frequency fluctuations of two completely independent ring dye lasers locked to independent reference cavities. Single laser linewidths of less than 750 Hz were achieved, the principal limitation being residual vibrations from the noisy laboratory environment. With future design and environmental improvements, ultranarrow linewidths are expected thus providing a useful tool for a great variety of high precision experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688526
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  • 6
    Electronic Resource
    Electronic Resource
    Immunological detection and localization of a calsequestrin-like protein in red beet and cucumber cells (1994)
    Springer
    Protoplasma 179 (1994), S. 158-165 
    Details
    ISSN: 1615-6102
    Keywords: Beta vulgaris ; Calcium binding ; Calsequestrin ; Cucumis sativus ; Endoplasmic reticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Calsequestrin is a calcium binding protein present in the sarcoplasmic reticulum (SR) of animal muscle cells and is thought to be essential for the rapid uptake and release of Ca2+, and thus for the regulation of Ca2+-dependent cellular functions. Higher plant cells of red beet (Beta vulgaris L.) and cucumber (Cucumis sativus L.) contain a polypeptide of about Mr 55000 that cross-reacts with a monoclonal antibody raised against calsequestrin from rabbit skeletal muscle SR. In beet this protein changes its apparent molecular weight with pH as indicated in Western immunoblotting. Although this protein bound calcium it was not the dominant calcium-binding protein in red beet. Washing of beet root tissue leads to a slight increase of this polypeptide in microsomal fractions as indicated by immunoblotting. After immunoblotting to partially purified cell membrane fractions this polypeptide appeared to be predominantly associated with endoplasmic reticulum-enriched fractions. Immunogold labelling of ultrathin sections of cucumber hypocotyl using the anti-calsequestrin antibody showed that gold particles were very largely confined to the cytosol and often in close proximity to the ER. Clusters of up to nine gold particles were observed, often over small vesicular areas, as observed in some animal tissues. These results indicate that red beet and cucumber cells contain a protein which may be related to animal calsequestrin. It appears to be associated with the ER and could be involved in cellular calcium regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403954
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of calcium antagonists and inhibitors of secretory processes on auxin-induced elongation and fine structure ofPisum sativum stem segments (1986)
    Springer
    Protoplasma 133 (1986), S. 149-159 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ; Cell elongation ; Indole-3-acetic acid ; Pisum sativum ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The treatment of dark grown pea stem segments with chelators of divalent cations (EGTA, EDTA, CTC), various Ca2+ antagonists (LaCl3, A-23187, verapamil) and inhibitors of secretory processes (monensin, CB) reduced elongation in the presence of indole-3-acetic acid (IAA). Generally the inhibition increased with increasing concentrations of the substances. The timing of the responses can be correlated with maximum auxin-stimulated secretion of cell wall material. Examination of cell ultrastructure showed that changes in dictyosome activity could explain a reduced deposition of cell wall material and so cause inhibition of elongation. The inhibitors affected the morphology and vesiculation of the dictyosomes, and the appearance of the plasma membrane, ER and mitochondria in different ways. The most pronounced effects on ultrastructure resulted from monensin and LaCl3 treatments with the dictyosomes being most affected; large vesicles appeared in the cytoplasm. Less pronounced effects on cell structure were seen in EGTA, A-23187 and verapamil treated tissue. The effects on the dictyosomes are considered to be due to disturbances of Ca2+ and other ionic levels within the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01304630
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  • 8
    Electronic Resource
    Electronic Resource
    The multigrid method in solid mechanics: Part II - Practical applications (1990)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 29 (1990), S. 739-753 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The performance of the multigrid algorithm is investigated by solving some large, practical, three dimensional solid mechanics problems. The convergence of the method is sensitive to factors such as the amount of bending present and the degree of mesh non-uniformity, as was also observed in Part I for two dimensional problems. However, in contrast to Part I, no proportionality is observed between the total number of operations to convergence and the problem size. Despite such behaviour, the multigrid algorithm proves to be an effective matrix equation solver for solid mechanics poblems. It is orders of magnitude faster than a direct factorization method, and yields converged solutions several times faster than the Jacobi preconditioned conjugate gradient method.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620290405
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  • 9
    Electronic Resource
    Electronic Resource
    The multigrid method in solid mechanics: Part I - Algorithm description and behaviour (1990)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 29 (1990), S. 719-737 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A multigrid algorithm is described that can be used to obtain the finite element solution of linear elastic solid mechanics problems. The method is applied to some two dimensional problems to evaluate its strengths and weaknesses. Extensive studies are made to determine the convergence behaviour of the method. In general, this depends on many factors: the number of degrees-of-freedom in the discretization, characteristics of the algorithm, Poisson's ratio when it is close to 0·5, the amount of bending deformation in the problem under consideration, and the degree of non-uniformity in the mesh. Only certain values of the multigrid parameters allow a converged solution to be obtained with a computational effort proportional to the number of degrees-of-freedom. These values include the optimum ones, i.e. those that lead to convergence with the least computational effort. The constant of proportionality is only independent of the number of degrees-of-freedom and still depends on the other factors listed above.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620290404
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