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  • 1
    Electronic Resource
    Electronic Resource
    Time averaging by means of a computer controlled dye-laser spectrometer (1980)
    Springer
    Applied physics 22 (1980), S. 361-364 
    Details
    ISSN: 1432-0630
    Keywords: 07.65 ; 42.80 ; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The noise characteristic of available laser sources limits the sensitivity of saturated absorption spectroscopy. Time averaging is one method to improve the signal-to-noise ratio. A computer controlled dye-laser spectrometer is described which has the capability to scan several times any selected frequency range with an absolute accuracy of ±200 kHz. The sensitivity of this system is demonstrated by measuring the isotope shift of the low abundant38Ar and by detecting a weak83Kr-hyperfine component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00901057
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  • 2
    Electronic Resource
    Electronic Resource
    Conformational changes of Na,K-ATPase probed with eosin Y (1996)
    Springer
    Journal of fluorescence 6 (1996), S. 165-168 
    Details
    ISSN: 1573-4994
    Keywords: Cation binding ; fluorescence decay ; kinetics ; binding constants ; Na,K-ATPase ; eosin Y
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Time-resolved fluorescence and binding studies have been carried out on Na,K-ATPase in the presence of the fluorescent dye eosin Y to obtain thermodynamic and kinetic parameters for the interaction of the enzyme with different cations. Eosin Y binding is indicated by a 3 ns fluorescence decay process and is observed only in the presence of mono- and divalent cations. This type of cation binding is interpreted as a nonselective electrostatic interaction, with negatively charged groups of the enzyme providing a high-affinity eosin Y binding site. Eosin Y binding is observed only under conditions where the enzyme exists in the conformational state F1. The kinetic parameters of eosin Y binding have been determined employing stopped-flow fluorometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00732056
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    DEDO: A specific, fluorescent inhibitor for spectroscopic investigations of Na,K-ATPase (1994)
    Springer
    Journal of fluorescence 4 (1994), S. 287-290 
    Details
    ISSN: 1573-4994
    Keywords: Na,K-ATPase ; fluorescent inhibitor ; kinetics ; energy transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The interaction between the fluorescent ouabain derivative DEDO and purified renal Na,K-ATPase (isolated from different animal species) is investigated. Equilibrium binding studies provide a pK value of about 7.5 and a stoichoimetric coefficient of 1. Nonmodified ouabain exhibits the same affinity to the rabbit enzyme; the enzyme originating from the other sources binds DEDO 10 times less strongly than ouabain. Kinetic studies indicate that this is the consequence of a 10 times higher dissociation rate constant of the complexes formed with DEDO. The fluorescence emission intensity of DEDO is enhanced, being dependent on the enzyme source. The single decay time of DEDO is 3 ns in the absence and 21 ns in the presence of the rabbit enzyme and 14 ns in the presence of the pig renal enzyme. This result suggests that the fluorophore of DEDO is bound to a very hydrophobic environment of the enzyme. Further characterization of the static fluorescence spectra provides evidence for energy transfer between Trp residues of the enzyme and DEDO. Distance estimations suggest that one or two Trp residues are likely to be located in the proximity of the fluorophore.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01881441
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  • 4
    Electronic Resource
    Electronic Resource
    Zur Anwendung der paramagnetischen Elektronenresonanz in der organischen Chemie (1965)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 77 (1965), S. 888-900 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Anwendungsmöglichkeiten der paramagnetischen Elektronenresonanz (EPR) in der organischen Chemie sind durch neue Methoden zur Radikalerzeugung, wie Belichten, Elektrolysieren oder Ablaufenlassen von Startreaktionen in Durchflußsystemen wesentlich erweitert worden. Neuere Arbeitsgebiete sind die Untersuchung von Triplett-Zuständen, Donator-Acceptor-Komplexen, kurzlebigen Radikalzwischenstufen und Reaktionskinetiken.  -  Es werden die Grundlagen der für eine optimale Auswertung von EPR-Spektren erforderlichen molekültheoretischen Berechnungen von Radikalen mit π-Elektronensystemen skizziert. Für kompliziertere Moleküle bewähren sich bei den Autoren erhältliche Programme für elektronische Rechner. Aus den EPR-Meßdaten Intensität, Linienbreite, Hyperfeinstruktur-Aufspaltung und g-Faktor lassen sich Radikalausbeuten, Ionenwertigkeiten, Rotationsfrequenzen in Rotameren, Elektronendichte-Verteilungen, Elektronegativitäten, innermolekulare Torsionswinkel, Lösungsmitteleinflüsse (infolge Wasserstoffbrücken) und π-Bahnenergien bestimmen.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19650772003
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  • 5
    Electronic Resource
    Electronic Resource
    The Use of Electron Paramagnetic Resonance in Organic Chemistry (1965)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 4 (1965), S. 856-867 
    Details
    ISSN: 0570-0833
    Keywords: EPR spectroscopy ; Free radicals ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The more recent applications of electron paramagnetic resonance (EPR) to organic chemistry include the investigation of triplet states, donor-acceptor complexes, short-lived free-radical intermediates, and reaction kinetics. The principles of the theoretical calculations required to obtain the maximum information from EPR spectra are outlined for free radicals containing π-electron systems. EPR intensities, line widths, hyperfine splitting, and g factors permit the determination of free-radical yields, ionic charges, rotation frequencies in rotamers, electron density distributions, electronegativities, intramolecular torsion angles, solvent effects (resulting from hydrogen bonding), and π-orbital energies. - The production of free radicals with the aid of initiation reaction in flow systems, by irradiation, and by electrolysis seems particularly promising.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.196508561
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