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  • 1
    Electronic Resource
    Electronic Resource
    Meiotic segregation and recombination of the intergenic spacer of the ribosomal DNA in the ectomycorrhizal basidiomycete Laccaria bicolor (1996)
    Springer
    Current genetics 30 (1996), S. 332-337 
    Details
    ISSN: 1432-0983
    Keywords: Key words Laccaria bicolor ; Basidiomycete ; Intergenic spacers ; Ribosomal DNA ; Meiotic recombination ; Heteroduplex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The aim of this study was to clarify the inheritance of the nuclear ribosomal DNA (rDNA) in the ectomycorrhizal basidiomycete Laccaria bicolor S238N in order to resolve inter- and within-strain relationships in forest ecosystems. PCR amplification of the intergenic spacer (IGS) was carried out in the dikaryotic mycelium and its haploid progeny. In the dikaryotic mycelium, multiple amplification products were produced for the 25s/5s (IGS1) and 5s/17s (IGS2) intergenic spacers. The 4.5- and 4.0-kb fragments of IGS2 (haplotypes α and β, respectively) were observed to occur in a 1:1 ratio within the haploid progeny as a result of divergent IGS haplotypes in the two separate nuclei. Recombinant monokaryons having both types of IGS2 occurred at a low frequency (6.5%; 60 kb per centimorgan) during meiosis. Haplotypes α and β of IGS1 cross-hybridized forming heteroduplexes during the PCR temperature cycle. The two IGS1 haplotypes differed only by the repeat number of a TA2C3 motif and co-segregated with the IGS2 haplotypes. Heteroduplex formation and IGS polymorphism provide information that is helpful in distinguishing between introduced exotic L. bicolor S238N and indigenous populations of Laccaria spp. in forest ecosystems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050141
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  • 2
    Electronic Resource
    Electronic Resource
    Total cross sections of reactions induced by neutron-rich light nuclei (1989)
    Springer
    The European physical journal 332 (1989), S. 457-465 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.-k ; 07.75.+h ; 21.10.Gv ; 25.70.Np ; 27.20.+n
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Total nuclear reaction cross-sections are determined by means of a 4π-γ method. The results cover a wide span of targets for various stable beams. The validity of the method is shown in a combined systematics including also the results of transmission-type experiments. The data are very well described by the formula developed by Kox et al. The same method is applied to secondary fragment beams produced from a 44 MeV/u22Ne beam on a 332mg/cm2 181Ta target. Using the LISE spectrometer the fragments4, 6He,6–9, 11Li,7, 9–12, 14Be,10–15, 17B11–19C,13–19N,15–21O,18– 21F and20,21 Ne are analyzed and transported to interact with a 199.4 mg/cm2 Cu target surrounded by a 4π-γ counter. The measured total reaction cross-sectionsσ R are discussed in terms of the reduced strong absorption radiusr 0 and compared with other experimental results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01292431
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    Paper (German National Licenses)
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