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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning, sequencing, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346 (2000)
    Springer
    Extremophiles 4 (2000), S. 115-122 
    Details
    ISSN: 1433-4909
    Keywords: Key words Uracil-DNA glycosylase ; Gene sequence ; Expression ; Psychrophilic bacterium ; Cold-active enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding a heat-labile uracil-DNA glycosylase (UDG) from a psychrophilic, gram-positive marine strain (BMTU3346) has been cloned, sequenced, and expressed in Escherichia coli. The UDG is a cold-active enzyme with an apparent temperature optimum of 35°C and a half-life of 2 min at 40°C. The amino acid sequence shows an identity of 39.1%–46.2% to UDGs from mesophilic bacteria. The primary structure was examined for features that could be related to the thermolability of the enzyme. The amino acid sequence of the heat-labile UDG shows 22 differences with respect to the consensus sequence derived from bacterial UDGs. Features previously recognized in cold-active enzymes such as extended surface loops or a decrease in the number of arginine residues or proline residues in loops were not observed. Because dominant features that could be related to the thermolability of the UDG from BMTU3346 cannot be identified, more subtle modifications of the conformation seem to be responsible for its thermolability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050145
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Prolongation of graft survival in allogeneic islet transplantation by (−) 15-deoxyspergualin in the rat (1987)
    Springer
    Diabetologia 30 (1987), S. 38-40 
    Details
    ISSN: 1432-0428
    Keywords: 15-Deoxyspergualin ; pancreatic islets ; graft survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of 15-Deoxyspergualin, a novel drug which has been described to have anti-tumour activity, on allogeneic graft survival (Dark Agouti ⇀ Lewis rats) after pancreatic islet transplantation was tested. A marked prolongation of graft survival could be shown using doses of 1.0, 2.5 and 5.0mg Deoxyspergualin/kg on day 0 until day +9 post transplantation. A maximum of 55.6 days (average) survival time was observed using 2.5mg/kg Deoxyspergualin compared to 5.2±0.6 days without immunosuppression. Using the chemiluminescence reaction of recipient monocytes after islet transplantation, a marked suppression of the monocyte system exceeding the treatment period could be observed. Since, in contrast to cyclosporin, B-cell toxicity could not be shown, the new drug seems to be a hopeful step towards successful allogeneic islet transplantation for treatment of diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01788905
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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