Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-2013
    Keywords: Cardiac muscle ; butanedione monoxime ; heat production ; aequorin luminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract At low concentrations (up to 5 mM) the compound 2,3-butanedione monoxime (BDM) was found to reduce twitch tension and initial heat production in isolated papillary muscles without significantly affecting the size of the intracellular Ca transient measured with aequorin luminescence. Higher concentrations of BDM caused further inhibition of twitch tension and heat production with a fall in the size of the Ca+ transient. The size of the aequorin transient was 50% of the control value at 15 mM BDM while twitch tension was negligible. These results suggest that BDM selectively inhibits Ca2+ activated force in cardiac muscle at low concentrations with additional effects on intracellular calcium at concentrations above 5 mM.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 421 (1992), S. 343-349 
    ISSN: 1432-2013
    Keywords: Cardiac muscle ; Rigor tension ; Ca2+ ; Caffeine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ventricular trabeculae from rat heart were chemically skinned with Triton-X100, which disrupts all cellular membranes including the sarcoplasmic reticulum. Trabeculae developed a maintained rigor contracture when adenosine triphosphate was withdrawn from the bathing medium. In all preparations, the final level of rigor force developed in the presence of caffeine (10–40 mM) was greater than under control conditions. However, caffeine failed to increase rigor tension when applied after contracture had fully developed. The effect of caffeine on rigor was maximal at about 15 mM; concentrations greater or less than 15 mM were less effective. On average, caffeine decreased the time required to develop half-maximum rigor force. The caffeine-induced potentiation of rigor force occurred in the effective absence of Ca2+ (10−9 M), in solutions strongly Ca2+-buffered with [ethylenebis(oxonitrilo)]tetraaceticacid (10–50 mM). In all preparations, rigor force was found to be independent of [Ca2+] over the range 10−10 M to about 10−7 M. These results suggest that caffeine affects rigor force by a direct effect on the myofilaments via a mechanism that is independent of Ca2+.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 147 (1992), S. 49-57 
    ISSN: 1573-5036
    Keywords: ammonium ; field ; kiwifruit ; 15N ; nitrogen fertilizer ; recovery ; soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fate of 15N-labelled ammonium fertilizer applied once to six-year-old field-grown kiwifruit (Actinidia deliciosa ‘Hayward’) vines was measured over three years. The three main treatments were nitrogen (N) applied singularly at 100 or 200 kg N ha−1 in early spring (two weeks before bud burst) or split with 100 kg N ha−1 (unlabelled) in early spring and 100 kg N ha−1 (15N-labelled) ten weeks later. All N treatments were applied to vines with a history of either 50 or 200 kg N ha−1 yr−1. For three years after 15n application, components of the vines and soil (0–600 mm depth) were sampled at harvest in late autumn and the N and 15N contents determined. By the first harvest, all plant uptake of 15N had occurred and this represented 48–53% of the 15N applied. There was no significant effect of current N fertilizer treatment or of N history on 15N recovery by vines. Removal of 15N in harvested fruit was small at 5–6% in the first year and 8% over 3 years. After 2–3 years, most plant 15N occurred in the roots and this component declined only slowly over time. In contrast, there was a large temporal decline in 15N in above-ground plant components due to the annual ‘removal’ in leaf fall and pruning. An associated experiment showed that when 15N-labelled prunings and leaves were mulched and returned to the soil, only about 9% was recovered by plants within 2 years. Almost all remaining mulched material had been immobilised into the soil organic N. In all treatments, about 20% of the added 15N remained in soil at the first harvest. This was almost entirely in organic fractions (〈0.4% in inorganic N) and mostly in the surface 150-mm layer. The 15N content in soil changed little over time (from 20 to 17% between the first and third harvests respectively) and indicated that most of the N had been immobilised into stable humus forms.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 147 (1992), S. 59-68 
    ISSN: 1573-5036
    Keywords: kiwifruit ; 15N ; plant uptake ; remobilisation ; temporal changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Temporal changes in the nitrogen (N) and 15N content of various components of six-year-old kiwifruit (Actinidia deliciosa ‘Hayward’) vines which had received 15N-labelled ammonium fertilizer were measured. The fertilizer was applied singularly at 100 or 200 kg N ha−1 in early spring (two weeks before bud burst) or split with 100 kg N ha−1 (unlabelled) in early spring and 100 kg N ha−1 (15N-labelled) ten weeks later. All treatments were applied to vines with a history of either 50 or 200 kg N ha−1 yr−1. The N concentration of leaf and fruit tissue was generally lower in the 100 kg N ha−1 treatment than in the 200 kg N ha−1 treatments and this effect was greater than that of N fertilizer history. During the first 8 weeks after bud burst there was a rapid accumulation of N in leaves (ca. 80 kg N ha−1). Analysis of xylem sap at 4 weeks after bud burst revealed that about 60% of the N utilised for new growth was from remobilisation of N stored within the vines and about 40% from soil and fertilizer N. This was unaffected by rate of N application. Plant uptake of added 15N was rapid and almost complete within 10 weeks of application in either early spring or early summer. Initially, most 15N was present in the leaf and root components but these subsequently declined due to translocation into other components of the vine. The decline in leaf 15N coincided with an equivalent accumulation of 15N in the fruit. The 15N enrichment of the ‘annual’ components (leaves, fruit and current-season's shoots) was about twice that of the structural components (one-year-old shoots, cordon, stem and structural roots) during the first year after application. By the third year the 15N concentration of the annual components had declined to similar levels to that of the structural components. These changes were used to estimate the annual throughput of N from soil in vines which received no N fertilizer in years 2 and 3 at 50% of total above-ground N and only 7% in roots. This was equivalent to about 120 kg N ha−1 yr−1. Where fertilizer N (200 kg ha−1) was applied in year 2 the annual throughput of N from soil and fertilizer was about 170 kg N ha−1.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Basic research in cardiology 88 (1993), S. 421-429 
    ISSN: 1435-1803
    Keywords: Cardiac muscle ; hypoxia ; pH ; lactate ; ischaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary During hypoxia cardiac contraction declines and there is an intracellular acidosis. We find that, if this acidosis is abolished by decreasing pCO2 there is little restoration of force. Therefore the acidosis is not the major cause of the decline of force. The acidosis may partly result from the generation of lactic acid. No acidosis, is however, seen in isolated cardiac cells. Furthermore a theoretical model shows that lactic acid production would be expected to produce a transient acidosis. We suggest that the observed maintained acidosis may be a consequence of extracellular lactic acid accumulation affecting intracellular pH.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...