ISSN:
1612-1112
Keywords:
Amino acids
;
Plasma
;
High-pressure liquid chromatography
;
2,4-Dinitrophenylderivatives
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The derivatization reaction of human plasma was performed with 2,4-dinitrofluorobenzen at 40 °C during 3 hours. Separation occurred on reverse-phase C-18 columns, 50 min were necessary to achieve complete elution. Exactitude, precision, sensitivity of the method were better than the conventional ion-exchange separation and ninhydrin assay procedure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02259151
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