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  • Chemistry  (9)
  • Minisatellite  (3)
  • 21.10.Dr  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 942-949 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza ; Rice ; PCR ; DNA fingerprinting ; Minisatellite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050645
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  • 2
    Electronic Resource
    Electronic Resource
    Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell) amplified with minisatellite core sequences (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 982-989 
    Details
    ISSN: 1432-2242
    Keywords: Genome-specific ; DAMD ; Minisatellite ; PCR ; Triticum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored the direct amplification of minisatellite DNA by PCR (DAMD-PCR) as a means for generating DNA probes that are useful for detecting DNA polymorphisms and DNA fingerprinting in wheat. This technique was facilitated by high-stringency PCR with known plant and animal minisatellite core sequences as primers on wheat genomic DNA. The products of DAMD-PCR from Triticum aestivum, T. durum, T. monococcum, T. speltoides and T. tauschii showed a high degree of polymorphism and the various genomes could be identified. Cloning of the DAMD-PCR products and subsequent Southern hybridization frequently revealed polymorphic probes showing a good degree of genome specificity. In addition, polymorphic, single locus, and moderately dispersed PCR products were cloned that may have a potential for DNA fingerprinting. Our experiments were limited primarily to diploid wheats and the results indicated that DAMD-PCR may isolate genome-specific probes from wild diploid wheat species that could be used to monitor genome introgression into hexaploid wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224102
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  • 3
    Electronic Resource
    Electronic Resource
    PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 276-283 
    Details
    ISSN: 1432-2242
    Keywords: Key words PCR ; Minisatellite ; DNA fingerprinting ; Wheat ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Four minisatellite core sequences were used as primers in a polymerase chain reaction (PCR) technique, known as the directed amplification of minisatellite-region DNA (DAMD), to detect polymorphisms in three pairs of hexaploid/tetraploid wheat cultivars. In each pair, the tetraploid cultivar (genomic formula AABB) was extracted from its corresponding hexaploid (genomic formula AABBDD) parent. Reproducible profiles of the amplified products revealed characteristic bands that were present only in the hexaploid wheats but not in their extracted tetraploids. Some polymorphisms were observed among the hexaploid cultivars. Twenty-three DAMD-PCR amplified fragments were isolated and screened as molecular probes on the genomic DNA of wild wheat species, hexaploid wheat and triticale cultivars. Subsequently, 8 of the fragments were cloned and sequenced. The DAMD-PCR clones revealed various degrees of polymorphism among different wild and cultivated wheats. Two clones yielded individual-specific DNA fingerprinting patterns which could be used for species differentiation and cultivar identification. The results demonstrated the use of DAMD-PCR as a tool for the isolation of informative molecular probes for DNA fingerprinting in wheat cultivars and species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050560
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  • 4
    Electronic Resource
    Electronic Resource
    Direct mass measurements of the neutron-rich isotopes of chlorine through iron (1990)
    Springer
    The European physical journal 337 (1990), S. 361-366 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.Dr ; 27.40.+z ; 27.50.+e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Direct mass measurements of up to 6 isotopes for each of the elements chlorine through iron (over 35 masses) have been performed using the Time-of-Flight Isochronous (TOFI) spectrometer. We have used these measurements to: (1) investigate the local dependence of neutron and proton pairing energies on neutron excess, (2) find a new region of enhanced binding centered around53Sc, and (3) evaluate the predictive capabilities of several mass models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294971
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  • 5
    Electronic Resource
    Electronic Resource
    Mass measurement of neutron-rich isotopes from51Ca to72Ni (1994)
    Springer
    The European physical journal 349 (1994), S. 25-32 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.Dr ; 27.40.tz ; 27.50.+ e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The ground state masses of thirty-nine neutronrich nuclei from51Ca to72Ni have been measured using the Time-of-Flight Isochronous (TOFI) spectrometer. Eight of these masses have been measured for the first time and thirty-one are remeasurements of neutron-rich nuclei previously reported. Good agreement between these results and a previous TOFI experiment was observed except for the most neutron-rich isotopes of vanadium through iron with the present results being more bound and in better agreement with theory. The low binding energy of68Ni, as indicated by an unreasonably low two-neutron separation energy, suggests the presence of a high-lying, long-lived isomeric state in this nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01296329
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  • 6
    Electronic Resource
    Electronic Resource
    Gas sorption in poly(butylene terephthalate). A critical test of the influence of molecular gas dataDedicated to Prof. Dr. D. Braun on the occasion of his 60th birthday (1991)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 185 (1991), S. 265-274 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: To test the molecular parameters concerning gas sorption in polymers, the concentration of CO2, N2O, CO, N2, CH4 and the noble gases Ne, He in glassy poly(butylene terephthalate) films (PBTP) has been studied gravimetrically with a recording microbalance at 25°C. The sorption isotherms exhibit downward curvature to the pressure axis. As neither solubility nor adsorption can explain the experimental results, analysis was carried out based on the dual-sorption model: gas dissolution and microvoid filling are considered as independent sorption mechanisms. The parameters of the dual-sorption model for the mentioned penetrants are determined. The results indicate that for parameter correlation the Lennard-Jones potential parameters give a rough idea, but size exclusion of gases in small diameter microvoids is proposed and special chemical interactions must be considered.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1991.051850125
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  • 7
    Electronic Resource
    Electronic Resource
    J-R curve calculation with the normalization method for toughened polymers (1994)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 34 (1994), S. 128-134 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The method of normalization is used to develop the J-R curve fracture toughness characterization for polymeric materials. This method can develop J-R curves directly from load vs. displacement records without a need for an on-line crack monitoring system. It was used previously to develop J-R curves for metallic materials and is applied here for the first time to polymer materials. Single edge notched bend specimens of rubber toughened nylon 6/6 and rubber toughened amorphous nylon are used in this study. The J-R curves from the method of normalization are compared with the results obtained from the multiple specimen method of ASTM Standard E813. The results show that the method of normalization gives reasonable J-R curves; both methods show agreement over the early J-R curve region. In addition the JIc values are determined for each method and compared. Based on this work it is suggested that the method of normalization could be used as a general test method to develop J-R curves for polymeric materials.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760340209
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  • 8
    Electronic Resource
    Electronic Resource
    Cold-drawing (necking) behavior of polycarbonate as a double glass transition (1995)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 304-309 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The thermomechanical behavior of poly(bisphenol A carbonate) (PC) undergoing cold-drawing (necking) over a large range of temperature and strain rate has been studied. The cold-drawing of PC has been described from a material particle perspective in terms of true stress and strain relationships. The isothermal draw stress is shown to be a material parameter, and the true stress-strain behavior of necked material above the true drawing stress follows conventional treatment by rubber elasticity. Cold-drawing is described as a double glass transition: first, a transition from an isotropic glass to an isotropic rubber at the yield point, then, on unloading after stretching of a rubbery mesophase, a transition from an oriented rubber to an oriented glass.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760350404
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  • 9
    Electronic Resource
    Electronic Resource
    Novel filled polymer composites prepared from in situ polymerization via a colloidal approach: I. Kaolin/Nylon-6 in situ composites (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 1761-1768 
    Details
    ISSN: 0887-6266
    Keywords: Filled composite ; colloidal polymerization ; kaolin ; Nylon-6 ; in-situ composite ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A mineral-filled in situ composite was prepared by a colloidal approach by first suspending kaolin filler particles in aqueous caprolactam, and then polymerizing caprolactam in situ at high pressure and temperature. The purpose of this colloidal in situ polymerization is to improve particle dispersion and to enhance interaction of the filler to the polymer matrix. X-ray diffraction studies of the in situ kaolin/Nylon-6 composites revealed that the x-ray peak corresponding to the α-crystal form of Nylon-6 diminished with increasing kaolin loading, while the γ-crystal structure became more pronounced. The degree of crystallinity of Nylon-6 remained fairly unchanged with the kaolin loading level in the in situ composites. Calorimetric and dynamic mechanical studies exhibited that the glass transition temperature of the resulting composite increased significantly with increase in kaolin concentration, suggesting strong filler-matrix interaction at the kaolin/Nylon-6 interface. Scanning electron microscopic (SEM) results showed uniform filler dispersion in the in situ composites relative to the conventional melt-mixed composites. Modulus and tensile strength of these in situ composites were found to be distinctively higher than that of the conventional melt-mixed kaolin/Nylon-6 composites. However, as typical for composite materials, drawability and fracture toughness decreased with increasing kaolin loading. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Novel filled polymer composites prepared from in situ polymerization via a colloidal approach: II. Blends of kaolin/Nylon-6 in situ composites with conventional Nylon-6 and -66 (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 1769-1775 
    Details
    ISSN: 0887-6266
    Keywords: in situ composite ; colloidal approach ; masterbatch ; kaolin ; nylon blends ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A novel in situ composite comprised of kaolin clay fillers and polyamide 6 (Nylon-6) was synthesized via a colloidal approach by suspending kaolin particles in aqueous caprolactam and then polymerizing the caprolactam under elevated temperature and pressure. This in situ polymerization technique enables the deposition of nylon molecules directly onto the filler surface. It offers a much larger contact surface area for the nylon molecules to interact with the filler particles and enhances filler/matrix interaction through polymer miscibility. The kaolin particles were shown to be uniformly dispersed in Nylon-6 matrix without appreciable agglomeration. In the highly clay-loaded composites such as the 50/50 kaolin/Nylon-6 in situ composite, the deposited nylon molecules probably form a coated layer on the filler particles. This kind of nylon coated fillers may be applied as a reinforcing entity to commercial Nylon-6 or -;66 by improving particle dispersion and melt processability. The 50/50 kaolin/Nylon-6 in situ composites have been used as a masterbatch for blending with commercial Nylon-6 and Nylon-66 to take advantage of their good properties and to reduce cost. Rheology and mechanical properties of the masterbatch/nylon composites have been investigated in comparison with those of the conventional melt-mixed composites. The improvement of rheological and mechanical properties of the in situ composites has been discussed in relation to the composite structure. © 1996 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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