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  • polymerase chain reaction  (3)
  • Achlys triphylla  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Four flavonol glycosides fromAchlys triphylla
    Amsterdam : Elsevier
    Phytochemistry 31 (1992), S. 301-303 
    Details
    ISSN: 0031-9422
    Schlagwort(e): Achlys triphylla ; Berberidaceae ; Epimedinae ; chemotaxonomy ; flavonol glycosides
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0031-9422(91)83058-S
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  • 2
    Digitale Medien
    Digitale Medien
    Four flavonol glycosides from Achlys triphylla
    Amsterdam : Elsevier
    Phytochemistry 31 (1991), S. 301-303 
    Details
    ISSN: 0031-9422
    Schlagwort(e): Achlys triphylla ; Berberidaceae ; Epimedinae ; chemotaxonomy. ; flavonol glycosides
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0031-9422(91)83058-S
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  • 3
    Digitale Medien
    Digitale Medien
    Abnormal messenger ribonucleic acid (mRNA) transcribed from a mutant insulin receptor gene in a patient with type A insulin resistance (1992)
    Springer
    Diabetologia 35 (1992), S. 639-644 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Insulin receptor ; type A insulin resistance ; deletion ; polymerase chain reaction ; insulin receptor gene ; direct sequence ; mRNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In a previous report on a 16-year-old Japanese girl with type A insulin resistance, we found that one allele of the insulin receptor gene was inherited from her mother and contained a 1.2 kilobase pair deletion which removed the 14th exon in the β subunit. We extended investigation of the proband and found the deletion between two Alu sequences. To determine the effect of the deletion on the level of transcription and the splicing pattern of messenger ribonucleic acid (mRNA), we synthesized the complimentary DNA and used the polymerase chain reaction to amplify the region which included the deleted area. The deletion shifted the reading frame, resulting in a termination codon after amino acid 867 (Glu), thereby producing a truncated insulin receptor without a transmembrane region and cytoplasmic domain. We also sequenced each of 22 exons of the insulin receptor gene but found no mutation in exons of the insulin receptor gene, except for deletion of exon 14 of the maternal allele. Thus, the proband is a heterozygote for a single mutant allele. Abnormal mRNA transcribed from the mutant allele resulted in a decrease in insulin binding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00400255
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  • 4
    Digitale Medien
    Digitale Medien
    Type 2 (non-insulin-dependent) diabetes mellitus associated with a mutation of the glucokinase gene in a Japanese family (1993)
    Springer
    Diabetologia 36 (1993), S. 433-437 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Glucokinase gene ; mutation ; Type 2 (non-insulin-dependent) diabetes mellitus ; polymerase chain reaction ; single stranded conformation polymorphism ; insulin secretion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Mutations were screened for in the glucokinase gene of 25 Japanese patients with Type 2 (non-insulin-dependent) diabetes mellitus. Each exon was scanned by electrophoresis of enzymatically amplified DNA segments under non-denaturing conditions and variants were sequenced. A variant pattern was detected in exon 5 of one patient. Direct sequencing of this exon revealed a single nucleotide substitution in codon 188 (GCT→ACT) of one of two alleles resulting in the mutation of Ala188→Thr, an invariant residue in the sequence of all mammalian glucokinases and hexokinases. This mutation was not found in 40 normal control subjects. The proband had been diagnosed with Type 2 diabetes at the age of 62 years. Four other members of her family have the same mutation and all have Type 2 diabetes or impaired glucose tolerance. The youngest age at diagnosis of Type 2 diabetes in these other members was 13 years, suggesting that her pedigree was maturity-onset diabetes of the young (MODY). All subjects with the Thr188 mutation show a decreased insulin secretory response during oral glucose tolerance testing. Mutations in the glucokinase gene associated with Type 2 diabetes have been previously identified in Caucasian (French and British) subjects. This study indicates that mutations in this gene are also implicated in the development of Type 2 diabetes in Asians. Further studies are required to determine the frequency of mutations in glucokinase among Japanese patients with Type 2 diabetes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00402280
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  • 5
    Digitale Medien
    Digitale Medien
    Expression of glycoprotein-processing enzymes in Escherichia coli as fusion proteins (1997)
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 265-267 
    Details
    ISSN: 1573-0972
    Schlagwort(e): β-1,4-galactosyltransferase ; fusion protein ; glycoprotein processing ; maltose binding protein ; man9-mannosidase ; polymerase chain reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A truncated Man9-mannosidase gene and a full-length β-1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018570604788
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