Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    A fungal chitinase gene fromRhizopus oligosporus confers antifungal activity to transgenic tobacco (1997)
    Springer
    Plant cell reports 16 (1997), S. 439-443 
    Details
    ISSN: 1432-203X
    Keywords: Fungal chitinase ; Transgenic tobacco ; Antifungal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied whether a chitinase involved in cell autolysis of a filamentous fungus,Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. Thechi1 gene was introduced into tobacco by theAgrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which thechi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogensSclerotinia sclerotiorum andBotrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092762
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A fungal chitinase gene from Rhizopus oligosporus confers antifungal activity to transgenic tobacco (1997)
    Springer
    Plant cell reports 16 (1997), S. 439-443 
    Details
    ISSN: 1432-203X
    Keywords: Key words Fungal chitinase ; Transgenic tobacco ; Antifungal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied whether a chitinase involved in cell autolysis of a filamentous fungus, Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. The chi1 gene was introduced into tobacco by the Agrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which the chi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogens Sclerotinia sclerotiorum and Botrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092762
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    TheAspergillus nidulans geneschsA andchsD encode chitin synthases which have redundant functions in conidia formation (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 442-450 
    Details
    ISSN: 1617-4623
    Keywords: Chitin synthase ; Multigene family ; Cell wall ; Conidia ; Aspergillus nidulans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We previously isolated three chitin synthase genes (chsA, chsB, andchsC) fromAspergillus nidulans. In the present work, we describe the isolation and characterization of another chitin synthase gene, namedchsD, fromA. nidulans. Its deduced amino acid sequence shows 56.7% and 55.9% amino acid identity, respectively, with Cal1 ofSaccharomyces cerevisiae and Chs3 ofCandida albicans. Disruption ofchsD caused no defect in cell growth or morphology during the asexual cycle and caused no decrease in chitin content in hyphae. However, double disruption ofchsA andchsD caused a remarkable decrease in the efficiency of conidia formation, while double disruption ofchsC andchsD caused no defect. Thus it appears thatchsA andchsD serve redundant functions in conidia formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172373
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Neutron-rich nuclei studied with AMD (1994)
    Springer
    The European physical journal 349 (1994), S. 279-283 
    Details
    ISSN: 1434-601X
    Keywords: 21.10 ; 24.10 ; 27.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Antisymmetrized molecular dynamics (AMD) which is a newly constructed theory of quantum-mechanical microscopic simulation of heavy ion collisions is explained to present us with a very useful theoretical approach for the study of the structure-changes which occur when we go from ordinary nuclei to exotic neutron-rich nuclei. It is because of the flexible nature of the AMD wave function which can describe the formation and dissolution of clusters both in collision problems and in structure problems. After brief explanation of successful applications of AMD to heavy ion collisions and that of the frictional cooling technique for constructing minimum-energy wave functions, the results of the AMD analyses of Li, Be, and B isotopes are discussed. Very good reproduction of magnetic moments is obtained and is explained to be related with the structure-change between cluster structure and shell-model-like structure within each isotope. The superposition of AMD determinants is shown to reproduce neutron halo structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01288975
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...